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We tested 277 remnant frozen nasopharyngeal swab samples, stored in universal transport medium (UTM), yielding a sensitivity of 94.9% against historical RT\u2010PCR results with cycle threshold (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>)\u2009&lt;\u200930, and a sensitivity of 76.7% for <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>\u2009&lt;\u200935, and specificity of 100% (all <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values) confirming compatibility of UTM\u2010diluted samples with the assay system. Thereafter, we prospectively collected 141 nasopharyngeal swab samples in UTM from healthcare workers and 1369 paired swabs (400 UTM; 969 dry) from individuals at a public health testing center, with the first swab (UTM) reserved for RT\u2010PCR, yielding a positivity rate of 4.6%. HISCL assay performance using UTM swabs was superior to dry swabs, with a sensitivity of 100% (95% confidence interval [CI] 71.5%\u2013100%) at <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>\u2009&lt;\u200930 versus 92.3% (95%CI 81.5%\u201397.9%), and a specificity of 99.3% (95% CI 98.1\u201399.89) against 83.3% (95%CI 80.7%\u201385.6%). We conclude that this antigen assay is suitable for high throughput facilities where the primary indication for testing is to rule out infection with low RT\u2010PCR <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values (proxy for high viral loads) to curb viral spread.<\/jats:p>","DOI":"10.1002\/jmv.27679","type":"journal-article","created":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T23:20:05Z","timestamp":1645831205000},"page":"3070-3080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A method comparison study of the high throughput automated HISCL\u00ae SARS\u2010CoV\u20102 antigen assay using nasopharyngeal swab samples from symptomatic and asymptomatic subjects against conventional RT\u2010PCR"],"prefix":"10.1002","volume":"94","author":[{"given":"Joachim","family":"Linssen","sequence":"first","affiliation":[{"name":"Sysmex Europe GMBH Norderstedt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Schapendonk","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases Radboud University Medical Center Nijmegen The Netherlands"},{"name":"Business Unit Microbiology Novel Foods &amp; Agrochains, Wageningen Food Safety Research (WFSR)  Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5366-4842","authenticated-orcid":false,"given":"Marion","family":"M\u00fcnster","sequence":"additional","affiliation":[{"name":"Sysmex Europe GMBH Norderstedt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Daemen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janette","family":"Rahamat\u2010Langendoen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases Radboud University Medical Center Nijmegen The Netherlands"},{"name":"Department of Viroscience Erasmus Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heiman","family":"Wertheim","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"volume-title":"BMJ Global Health","year":"2020","author":"Ghandour HHS","key":"e_1_2_10_2_1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"volume-title":"Diagnostic Detection of 2019\u2010nCoV by Real\u2010Time RT\u2010PCR","year":"2020","author":"Corman VBT","key":"e_1_2_10_4_1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00461-z"},{"volume-title":"Emergency Use Listing for In vitro diagnostics (IVDs) Detecting SARS\u2010CoV\u20102","year":"2021","author":"WHO","key":"e_1_2_10_6_1"},{"key":"e_1_2_10_7_1","unstructured":"FDA.In Vitro Diagnostics EUAs \u2010 Molecular Diagnostic Tests for SARS\u2010CoV\u20102. 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