{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:10:50Z","timestamp":1790766650288,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>SARS\u2010CoV\u20102 has evolved into a panel of variants of concern (VOCs) and constituted a sustained threat to global health. The wildtype (WT) SARS\u2010CoV\u20102 isolates fail to infect mice, while the Beta variant, one of the VOCs, has acquired the capability to infect standard laboratory mice, raising a spreading risk of SARS\u2010CoV\u20102 from humans to mice. However, the infectivity and pathogenicity of other VOCs in mice remain not fully understood. In this study, we systematically investigated the infectivity and pathogenicity of three VOCs, Alpha, Beta, and Delta, in mice in comparison with two well\u2010understood SARS\u2010CoV\u20102 mouse\u2010adapted strains, MASCp6 and MASCp36,\u00a0sharing key mutations in the receptor\u2010binding domain (RBD) with Alpha or Beta, respectively. Our results showed that the Beta variant had the strongest infectivity and pathogenicity among the three VOCs, while the Delta variant only caused limited replication and mild pathogenic changes in the mouse lung, which is much weaker than what the Alpha variant did. Meanwhile, Alpha showed comparable infectivity in lungs in comparison with MASCp6, and Beta only showed slightly lower infectivity in lungs when compared with MASCp36. These results indicated that all three VOCs have acquired the capability to infect mice, highlighting the ongoing spillover risk of SARS\u2010CoV\u20102 from humans to mice during the continued evolution of SARS\u2010CoV\u20102, and that the key amino acid mutations in the RBD of mouse\u2010adapted strains may be referenced as an early\u2010warning indicator for predicting the spillover risk of newly emerging SARS\u2010CoV\u20102 variants.<\/jats:p>","DOI":"10.1002\/jmv.27735","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T22:48:28Z","timestamp":1648075708000},"page":"3223-3232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Comparative characterization of SARS\u2010CoV\u20102 variants of concern and mouse\u2010adapted strains in mice"],"prefix":"10.1002","volume":"94","author":[{"given":"Qi","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of 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