{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:52:31Z","timestamp":1792097551460,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>With deep sequencing of virus genomes within the hosts, intrahost single nucleotide variations (iSNVs) have been used for analyses of virus genome variation and evolution, which is indicated to correlate with viral pathogenesis and disease severity. Little is known about the features of iSNVs among DNA viruses. We performed the epidemiological and laboratory investigation of one outbreak of adenovirus. The whole genomes of viruses in both original oral swabs and cell\u2010cultured virus isolates were deeply sequenced. We identified 737 iSNVs in the viral genomes sequenced from original samples and 46 viral iSNVs in cell\u2010cultured isolates, with 33 iSNVs shared by original samples and cultured isolates. Meanwhile, we found these 33 iSNVs were shared by different patients, among which, three hot spot areas 6367\u22126401, 9213\u22129247, and 10\u2009584\u221210\u2009606 within the functional genes of the adenovirus genome were found. Notably, the substitution rates of iSNVs were closely correlated with the clinical and immune indicators of the patients. Especially a positive correlation to neutrophils was found, indicating a predictable biomarker of iSNV dynamics. Our findings demonstrated the neutrophil\u2010correlated dynamic evolution features of the iSNVs within adenoviruses, which indicates a virus\u2212host interaction during human infection of a DNA virus.<\/jats:p>","DOI":"10.1002\/jmv.27744","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T22:49:42Z","timestamp":1648680582000},"page":"3863-3875","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intrahost variation and evolutionary dynamics of adenoviruses correlate to neutrophils in infected patients"],"prefix":"10.1002","volume":"94","author":[{"given":"Shuting","family":"Huo","sequence":"first","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hai","sequence":"additional","affiliation":[{"name":"Inner Mongolia Center for Disease Control and Prevention Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Guo","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Nie","sequence":"additional","affiliation":[{"name":"Tongliao Center for Disease Control and Prevention Tongliao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwen","family":"Qiao","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Zong","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Guo","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"},{"name":"School of Pharmaceutical Sciences Nanjing Tech University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingdong","family":"Song","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"},{"name":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglan","family":"Zhao","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Lei","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lan","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George F.","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou China"},{"name":"NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"},{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"Molecular epidemiology and phylogenetic analysis of human adenovirus caused an outbreak in Taiwan during 2011","volume":"10","author":"Lin YC","year":"2015","journal-title":"PLoS ONE"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06523-11"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.24.15265-15276.2005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-84-20714"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/522188"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1185-x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1584923"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3651-2"},{"key":"e_1_2_9_10_1","first-page":"15011","article-title":"Molecular epidemiology of respiratory adenovirus detection in hospitalized children in Shenzhen, China","volume":"8","author":"Wang H","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152324"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1007-4376(07)60071-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2308-8"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19790"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001553"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-021-00847-5"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146251"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bsheal.2019.02.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14490"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2013267117"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004052"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix120"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw044"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006738"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2021.100254"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9604-9608.1999"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01335-16"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10752-6"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90310-5"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.12.014"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90460-5"},{"key":"e_1_2_9_35_1","first-page":"1","article-title":"An outbreak of acute respiratory disease caused by a virus associated RNA II gene mutation strain of human adenovirus 7 in China, 2015","volume":"12","author":"Yang X","year":"2017","journal-title":"PLoS ONE"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.5.1240"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11681-11689.2000"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90145-8"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.17.5225"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2398"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2006.08.051"},{"key":"e_1_2_9_42_1","first-page":"79","article-title":"Adenoviral diseases in children: a study of 105 hospital cases","volume":"76","author":"Ruuskanen O","year":"1985","journal-title":"Pediatrics"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2011-301308"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.2310\/6650.2005.53605"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004696"},{"key":"e_1_2_9_46_1","first-page":"400","article-title":"Can we predict the severity of coronavirus disease 2019 with a routine blood test","volume":"130","author":"Zeng F","year":"2020","journal-title":"Pol Arch Intern Med"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.3331"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.32.1.206"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04388"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01801-15"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27744","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27744","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27744","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:50:15Z","timestamp":1771246215000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,11]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/jmv.27744"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27744","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,11]]},"assertion":[{"value":"2022-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}