{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T08:30:41Z","timestamp":1780475441883,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Coxsackievirus A10 (CV\u2010A10) is a major pathogen that causes hand, foot, and mouth disease. There are no effective therapeutic drugs for CV\u2010A10 infection; therefore, CV\u2010A10 vaccines should be developed. Previously, we isolated a CV\u2010A10 strain (N25) that can be cultured on Vero cells. In this study, the N25 strain was plaque\u2010purified three times from Vero cells, and three clones were selected for adaptive culture. The three clones of the 5th, 12th, and 19th generations were compared and analyzed in terms of viral titers, plaque morphology, pathogenicity in suckling mice, and nucleotide and amino acid sequences of the complete genome. The infectivity titers of the three clones (P2\u2013P22) were maintained at 6.5\u20137.0\u2009lgCCID\n                    <jats:sub>50<\/jats:sub>\n                    \/ml. The three clones began to proliferate at 6\u2009h and peaked at 36\u2009h; the corresponding CCID\n                    <jats:sub>50<\/jats:sub>\n                    was in the range of 10\n                    <jats:sup>6.5<\/jats:sup>\n                    \u201310\n                    <jats:sup>6.875<\/jats:sup>\n                    \/ml, which gradually decreased. The suckling mice in the challenged group exhibited clinical symptoms such as paralysis of the limbs, which gradually worsened until death. The inactivated vaccines prepared using the three clones efficiently induced antigen\u2010specific serum antibodies in mice. There were eight nucleotide mutations in the three clones, which resulted in two and four amino acid substitutions in the VP3 and VP1 coding regions, respectively. The nucleotide and amino acid sequence homology between the three clones and N25 were 99.92%\u2013100% and 99.78%\u2013100%, respectively, indicating high genetic stability. Our findings provide a theoretical basis for screening CV\u2010A10 vaccine candidate clones.\n                  <\/jats:p>","DOI":"10.1002\/jmv.27796","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T21:38:56Z","timestamp":1650317936000},"page":"3820-3828","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative analysis of the biological characteristics of three CV\u2010A10 clones adaptively cultured on Vero cells"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4762-7252","authenticated-orcid":false,"given":"Hong","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yue","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhong","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Xiang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingchen","family":"Wei","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongping","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academic Medical Sciences and Peking Union Medical College Kunming China"},{"name":"Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2019.1585242"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1712.110395"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2010.07.006"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.02.002"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20205201"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03789.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104516"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-04992-x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01231-13"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084233"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2017.05.011"},{"issue":"8","key":"e_1_2_11_13_1","first-page":"594","article-title":"Hand, foot and mouth disease","volume":"32","author":"Frydenberg A","year":"2003","journal-title":"Aust Fam Physician"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.9991"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1476-7"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.52.1042"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.12.011"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.02.008"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2020.07.003"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.06.093"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.02.016"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00333-17"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0094-1"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1304923"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.06.008"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27796","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27796","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27796","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T13:04:23Z","timestamp":1771247063000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27796"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,9]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/jmv.27796"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27796","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,9]]},"assertion":[{"value":"2021-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}