{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:15:26Z","timestamp":1740104126616,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Destruction of all poliovirus containing materials, safe and secure handling of retained polioviruses for vaccine production, and research will be obligatory to eliminate facility\u2010associated risks. Polioviruses and poliovirus potentially infectious materials (PIM) including fecal or respiratory samples requiring containment have been defined in World Health Organization\u2010Global Action Plan\u00a0(GAP III) documents. Non\u2010polio laboratories culturing viruses from PIM are most affected as cell cultures of human and monkey origin are also poliovirus permissive.\u00a0CRISPR gene\u2010editing technology was used to knockout the poliovirus receptor (PVR\/CD155) gene in the\u00a0rhabdomyosarcoma\u00a0(RD) cell line. PVR knockout RD cell susceptibility was tested using known non\u2010polio enterovirus (NPEV) types. A selected clone (RD\u2010SJ40) was field evaluated for virus isolation from 626 stool samples of acute flaccid paralysis cases. Poliovirus nonpermissive cells derived from the\u00a0RD cell line did not show CD155\u2010specific cell\u2010surface immunofluorescence. CD155 gene sequencing confirmed nucleotide base pair deletions within exon2 and exon3. The CD155 knockout RD\u2010SJ40 cells did not support the growth of poliovirus from positive stool samples. All NPEV types were isolated in RD and RD\u2010SJ40 cells.\u00a0CRISPR correctly edited the\u00a0CD155 gene of RD cells to render them poliovirus nonpermissive while susceptibility to NPEV remained unchanged. RD\u2010SJ40 cells are safe for NPEV isolation from poliovirus PIM without derogating GAP III containment requirements.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.27897","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T09:42:04Z","timestamp":1654076524000},"page":"4901-4909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Poliovirus nonpermissive CD155 knockout cells derived from RD cell line for handling poliovirus potentially infectious materials in virology laboratories"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-7199","authenticated-orcid":false,"given":"Shyam S.","family":"Nandi","sequence":"first","affiliation":[{"name":"National Institute of Virology Mumbai Unit Haffkine Institute Compound Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonali","family":"Sawant","sequence":"additional","affiliation":[{"name":"National Institute of Virology Mumbai Unit Haffkine Institute Compound Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trupti","family":"Gohil","sequence":"additional","affiliation":[{"name":"National Institute of Virology Mumbai Unit Haffkine Institute Compound Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upendra","family":"Lambe","sequence":"additional","affiliation":[{"name":"National Institute of Virology Mumbai Unit Haffkine Institute Compound Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucky","family":"Sangal","sequence":"additional","affiliation":[{"name":"WHO\u2010National Polio Surveillance Project New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Disha","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Microbiology National Polio Laboratory, B. J. Medical College Ahmedabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaveri","family":"Krishnasamy","sequence":"additional","affiliation":[{"name":"Department of Virology King Institute of Preventive Medicine and Research Guindy Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ujjala","family":"Ghoshal","sequence":"additional","affiliation":[{"name":"Department of Microbiology Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS) Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pauline","family":"Harvey","sequence":"additional","affiliation":[{"name":"WHO\u2010National Polio Surveillance Project New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jagadish","family":"Deshpande","sequence":"additional","affiliation":[{"name":"National Institute of Virology Mumbai Unit Haffkine Institute Compound Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Global Polio Eradication Initiative. Two out of three wild poliovirus strains eradicated. Accessed March 16 2020.http:\/\/polioeradication.org\/news-post\/two-out-of-three-wild-poliovirus-strains-eradicated\/"},{"key":"e_1_2_11_3_1","unstructured":"Global Polio Eradication Initiative. Global eradication of wild poliovirus type 2 declared.\u00a0Accessed March 16 2020.\u00a0http:\/\/polioeradication.org\/news-post\/global-eradication-of-wild-poliovirus-type-2-declared\/"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6820a3"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2994218"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.09.015"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.8.4827-4835.2003"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(93)90022-J"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.20.7845"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07520.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.7.4460-4469.2005"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13301"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.109.5.475"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196909)24:3<520::AID-CNCR2820240313>3.0.CO;2-M"},{"key":"e_1_2_11_15_1","unstructured":"World Health Organization.Field guide for supplementary activities aimed at achieving polio eradication 1996 revision(WHO\/EPI\/GEN\/95.01 REV. 1); 1997."},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803008562"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199906)58:2<188::AID-JMV15>3.0.CO;2-H"},{"volume-title":"Polio Laboratory Manual","year":"2004","author":"World Health Organization","key":"e_1_2_11_18_1"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2919-2927.2003"},{"key":"e_1_2_11_20_1","unstructured":"World Health Organization. Global Polio Eradication Initiative.Polio endgame strategy 2019\u20102023: eradication integration certification and containment;2019."},{"issue":"24","key":"e_1_2_11_21_1","first-page":"649","article-title":"Global polio eradication: progress towards containment of poliovirus type 2, worldwide 2017","volume":"66","author":"Previsani N","year":"2017","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_11_22_1","unstructured":"World Health Organization2015. WHO global action plan to minimize poliovirus facility\u2010associated risk after type\u2010specific eradication of wild polioviruses and sequential cessation of oral polio vaccine use: GAPIII (WHO\/POLIO\/15.05); 2015."},{"key":"e_1_2_11_23_1","unstructured":"World Health Organization.GAPIII containment certification scheme(GAPIII Annex 2 and Annex 3); 2015."},{"key":"e_1_2_11_24_1","unstructured":"World Health Organization.Poliovirus containment:\u00a0guidance to minimize risks for facilities collecting handling or storing materials potentially infectious for polioviruses(WHO\/POLIO\/18.03); 2018."},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.010"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1231143"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00542-06"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10360"},{"volume-title":"Guidelines for Environmental Surveillance of Poliovirus Circulation","year":"2003","author":"World Health Organization","key":"e_1_2_11_29_1"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/35022579"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4399-4401.2001"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.3.321"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01560-2"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90690-9"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.023960-0"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1179555"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232033"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2016.198"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.1.73"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27897","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27897","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T16:28:02Z","timestamp":1692721682000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,8]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/jmv.27897"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27897","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2022,6,8]]},"assertion":[{"value":"2021-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}