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The capsid protein plays crucial roles in ZIKV replication and thus represents an attractive therapeutic target. However, inhibitors of ZIKV capsid assembly have not been rigorously identified due to the lack of a target\u2010based screening system. In this study, we developed a novel ZIKV capsid interaction method based on a split\u2010luciferase complementation assay, which can be used to measure and quantify ZIKV capsid\u2013capsid (C\u2013C) interaction by the restored luciferase signal when capsid proteins interact with each other. Furthermore, a Tet\u2010on inducible stable cell line was generated to screen inhibitors of capsid dimerization. By using of this system, peptides (Pep.15\u201024 in the N\u2010terminal region of ZIKV capsid protein and Pep.44\u201058 in the \u03b12 helix of ZIKV capsid protein) were identified to inhibit ZIKV C\u2013C interaction. Overall, this study developed a novel inducible assay system to measure ZIKV capsid interaction and identify ZIKV capsid multimerization inhibitors, which will be applied for future discovery of ZIKV assembly inhibitors.<\/jats:p>","DOI":"10.1002\/jmv.27991","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T06:59:40Z","timestamp":1657695580000},"page":"5392-5400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and characterization of an inducible assay system to measure Zika virus capsid interactions"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1466-1339","authenticated-orcid":false,"given":"Jieshi","family":"Yu","sequence":"first","affiliation":[{"name":"Maxwell H. 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