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Accordingly, we developed a reusable dual\u2010channel optical fiber immunosensor (DOFIS), which utilized the evanescent wave\u2010sensing properties and tandem detection mode of the mobile phase, effectively accelerating the detection process such that it can be completed within 10\u2009min. It could detect the nucleoprotein of multiple influenza A viruses (H1N1, H3N2, and H7N9), as well as the spike proteins of the SARS\u2010CoV\u20102 Omicron and Delta variants, and could respond to 20\u2009TCID<jats:sub>50<\/jats:sub>\/ml SARS\u2010CoV\u20102 pseudovirus and 100\u2009TCID<jats:sub>50<\/jats:sub>\/ml influenza A (A\/PR\/8\/H1N1), presenting lower limit of detection\u00a0and wider linear range than enzyme\u2010linked immunosorbent assay. The detection results on 26 clinical samples for SARS\u2010CoV\u20102 demonstrated its specificity (100%) and sensitivity (94%), much higher than the sensitivity of commercial colloidal gold test strip (35%). Particularly, DOFIS might be reused more than 80 times, showing not only cost\u2010saving but also potential in real\u2010time monitoring of the pathogenic viruses. Therefore, this newly\u2010developed DOFIS platform is low cost, simple to operate, and has broad spectrum detection capabilities for SARS\u2010CoV\u20102 mutations and multiple influenza A strains. It may prove suitable for deployment as a rapid on\u2010site screening and surveillance technique for infectious disease.<\/jats:p>","DOI":"10.1002\/jmv.28015","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T03:24:16Z","timestamp":1658373856000},"page":"5325-5335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Rapid and universal detection of SARS\u2010CoV\u20102 and influenza A virus using a reusable dual\u2010channel optic fiber immunosensor"],"prefix":"10.1002","volume":"94","author":[{"given":"Yi","family":"Yang","sequence":"first","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9845-2405","authenticated-orcid":false,"given":"Rongtao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease 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