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In particular, Omicron contains a high number of mutations in the spike (S) protein gene, on which most vaccines were developed. In this study, we quantitated neutralizing antibodies in vaccine recipients at various times postvaccination using S protein\u2010based pseudoviruses derived from wild type (WT) SARS\u2010CoV\u20102 and five\u00a0VOCs including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (B.1.1.529). We found that two\u2010dose mRNA\u20101273 and BNT162b2 vaccines elicited robust neutralizing antibodies against WT, Alpha, Beta, Gamma, and Delta, but wanned after 6 months with a faster decline observed for BNT162b2. Both mRNA\u20101273 and BNT162b2 elicited weak neutralizing antibodies against Omicron. One dose of Ad26.COV2.S vaccine induced weaker neutralizing antibodies against WT and most VOCs than mRNA\u20101273 and BNT162b2 did but moderate neutralizing antibodies against Delta and Omicron, which lasted for 6 months. These results support current recommendations of the Centers for Disease Control and Prevention for a booster 5 months after full immunization with an mRNA\u2010based vaccine and the use of an mRNA\u2010based vaccine 2 months after Ad26.COV2.S vaccination.<\/jats:p>","DOI":"10.1002\/jmv.28032","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T22:34:51Z","timestamp":1659047691000},"page":"5678-5690","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Neutralizing antibodies to SARS\u2010CoV\u20102 variants of concern including Delta and Omicron in subjects receiving mRNA\u20101273, BNT162b2, and Ad26.COV2.S vaccines"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1316-523X","authenticated-orcid":false,"given":"George Fei","family":"Zhang","sequence":"first","affiliation":[{"name":"Cancer Virology Program, UPMC Hillman Cancer Center University of 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