{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:05:40Z","timestamp":1792004740469,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T00:00:00Z","timestamp":1662336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To rapidly identify individuals infected with severe acute respiratory syndrome coronavirus\u20102 (SARS\u2010CoV\u20102) and control the spread of coronavirus disease (COVID\u201019), there is an urgent need for highly sensitive on\u2010site virus detection methods. A clustered regularly interspaced short palindromic repeat (CRISPR)\/CRISPR\u2010associated protein (Cas)\u2010based molecular diagnostic method was developed for this purpose. Here, a CRISPR system\u2010mediated lateral flow assay (LFA) for SARS\u2010CoV\u20102 was established based on multienzyme isothermal rapid amplification, CRISPR\u2010Cas13a nuclease, and LFA. To improve the limit of detection (LoD), the crispr RNA, amplification primer, and probe were screened, in addition to concentrations of various components in the reaction system. The LoD of CRISPR detection was improved to 0.25 copy\/\u03bcl in both fluorescence\u2010 and immunochromatography\u2010based assays. To enhance the quality control of the CRISPR\u2010based LFA method, glyceraldehyde\u20103\u2010phosphate dehydrogenase was detected as a reference using a triple\u2010line strip design in a lateral flow strip. In total, 52 COVID\u201019\u2010positive and 101 COVID\u201019\u2010negative clinical samples examined by\u00a0reverse transcription polymerase chain reaction (RT\u2010PCR) were tested using the CRISPR immunochromatographic detection technique. Results revealed 100% consistency, indicating the comparable effectiveness of our method to that of RT\u2010PCR. In conclusion, this approach significantly improves the sensitivity and reliability of CRISPR\u2010mediated LFA and provides a crucial tool for on\u2010site detection of SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/jmv.28096","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T10:01:39Z","timestamp":1661594499000},"page":"5858-5866","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Highly sensitive and rapid detection of SARS\u2010CoV\u20102 via a portable CRISPR\u2010Cas13a\u2010based lateral flow assay"],"prefix":"10.1002","volume":"94","author":[{"given":"Hongbo","family":"Liu","sequence":"first","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuailei","family":"Chang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"},{"name":"Department of Epidemiology, College of Public Health Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Du","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"},{"name":"Department of Epidemiology, College of Public Health Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xiang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjun","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaofu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Song","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871526520666201109115820"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-06214-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.123409"},{"key":"e_1_2_10_6_1","unstructured":"deCamposASM deSouzaGAF deJesusRA et al.Main diagnostic methods for Covid\u201019: integrative review.Curr Pharm Des. 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