{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:22:39Z","timestamp":1790738559458,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The emergence and sustained transmission of novel pathogens are exerting an increasing demand on the diagnostics sector worldwide, as seen with the ongoing severe acute respiratory coronavirus 2\u00a0(SARS\u2010CoV\u20102) pandemic and the more recent public health concern of monkeypox virus (MPXV) since May 2022. Appropriate and reliable viral inactivation measures are needed to ensure the safety of personnel handling these infectious samples. In the present study, seven commercialized diagnosis buffers, heat (56\u00b0C and 60\u00b0C), and sodium dodecyl sulfate detergent (2.0%, 1.0%, and 0.5% final concentrations) were tested against infectious SARS\u2010CoV\u20102 and MPXV culture isolates on Vero cell culture. Cytopathic effects\u00a0were observed up to 7 days postinoculation and viral load evolution was measured by semiquantitative polymerase chain reaction.\u00a0The\u00a0World Health Organization recommends an infectious titer reduction of at least 4 log\n                    <jats:sub>10<\/jats:sub>\n                    . As such, the data show efficacious SARS\u2010CoV\u20102 inactivation by all investigated methods, with &gt;6.0 log\n                    <jats:sub>10<\/jats:sub>\n                    reduction. MPXV inactivation was also validated with all investigated methods with 6.9 log\n                    <jats:sub>10<\/jats:sub>\n                    reductions, although some commercial buffers required a longer incubation period to yield complete inactivation. These results are valuable for facilities, notably those without biosafety level\u20103\u00a0capabilities, that need to implement rapid and reliable protocols common against both SARS\u2010CoV\u20102 and MPXV.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28126","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T01:04:55Z","timestamp":1662944695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS\u2010CoV\u20102 and monkeypox virus"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8176-3955","authenticated-orcid":false,"given":"Gr\u00e9gory","family":"Qu\u00e9rom\u00e8s","sequence":"first","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Team VirPatH, Universit\u00e9 de Lyon, Inserm, U1111, CNRS, UMR5308,\u00a0ENS de Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Frobert","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Team VirPatH, Universit\u00e9 de Lyon, Inserm, U1111, CNRS, UMR5308,\u00a0ENS de Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon France"},{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maude","family":"Bouscambert\u2010Duchamp","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Oblette","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Valette","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Team VirPatH, Universit\u00e9 de Lyon, Inserm, U1111, CNRS, UMR5308,\u00a0ENS de Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon France"},{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"},{"name":"Centre National de R\u00e9f\u00e9rence des Virus des infections respiratoires (dont la grippe) Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Billaud","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Escuret","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Team VirPatH, Universit\u00e9 de Lyon, Inserm, U1111, CNRS, UMR5308,\u00a0ENS de Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon France"},{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"},{"name":"Centre National de R\u00e9f\u00e9rence des Virus des infections respiratoires (dont la grippe) Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Lina","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Team VirPatH, Universit\u00e9 de Lyon, Inserm, U1111, CNRS, UMR5308,\u00a0ENS de Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon France"},{"name":"Laboratoire de Virologie Institut des\u00a0Agents Infectieux, Hospices Civils de Lyon Lyon France"},{"name":"Centre National de R\u00e9f\u00e9rence des Virus des infections respiratoires (dont la grippe) Hospices 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization. Multi\u2010country outbreak of monkeypox external situation report #2\u2014July 25 2022.2022."},{"key":"e_1_2_8_3_1","unstructured":"Centers for Disease Control and Prevention. Monkeypox outbreak global map. 2022. Accessed July 28 2022.\u00a0https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world-map.html"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5501\/wjv.v5.i2.85"},{"key":"e_1_2_8_5_1","unstructured":"Association Fran\u00e7aise de Normalisation.NF EN 14476+A2-Antiseptiques et d\u00e9sinfectants chimiques \u2014 Essai quantitatif de suspension pour l'\u00e9valuation de l'activit\u00e9 virucide dans le domaine m\u00e9dical \u2014 M\u00e9thode d'essai et prescriptions(Phase 2\/\u00c9tape 1). AFNOR Editions;2019.https:\/\/viewerbdc.afnor.org\/html\/display\/T9-40j9fF341"},{"key":"e_1_2_8_6_1","unstructured":"World Health Organization. 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