{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:06:36Z","timestamp":1772982396751,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The present study was designed to check the serum levels of protease\u2010activated receptor (PAR\u20101) in patients during different phases of dengue severity. Moreover, a correlation between serum PAR\u20101 levels and hematological parameters, inflammatory cytokine levels, and liver functional changes was also determined. Based on the World Health Organization criteria, the study population was divided into: nonsevere dengue fever (DF;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200930), severe dengue hemorrhagic fever (DHF;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200919), and severe dengue shock syndrome (DSS;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911). The platelet count (PLT) and hematocrit (HCT) were analyzed using an automated hematology analyzer and liver function enzymes aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphate (ALP), bilirubin were checked by auto\u2010analyzer using diagnostic kits. Moreover, the levels of inflammatory mediators C\u2010reactive protein (CRP), tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interleukin\u20106 (IL\u20106), interleukin\u201017 (IL\u201017), and PAR\u20101 were determined using respective ELISA kits. The HCT levels were elevated and platelet count decreased significantly during dengue complications (DHF and\u00a0DSS) compared to the DF patients, while the levels of liver functional biomarkers AST, ALT, ALP, and bilirubin remained elevated in DHF and DSS groups than in the corresponding DF group. Similarly, the inflammatory cytokine levels of CRP, TNF\u2010\u03b1, IL\u20106, and IL\u201017 in DHF and DSS subjects were markedly increased when observed against DF subjects. Notably, the PAR\u20101 levels were significantly elevated in DHF and DSS groups than in the DF group and positively correlated with changes in HCT levels, inflammatory biomarkers, and liver enzymes. Our findings conclude that PAR\u20101 levels persistently increased with the severity of the dengue infection and are strongly associated with various clinical manifestations. Thus, PAR\u20101 levels can be used as a diagnostic marker for assessing dengue severity.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28152","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T22:22:45Z","timestamp":1663280565000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Elevated serum PAR\u20101 levels as an emerging biomarker of inflammation to predict the dengue infection severity"],"prefix":"10.1002","volume":"95","author":[{"given":"Raminderpal Singh","family":"Sibia","sequence":"first","affiliation":[{"name":"Government Medical College  Patiala Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankita","family":"Sood","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arshula","family":"Subedi","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anushya","family":"Sharma","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anirudh","family":"Mittal","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4032-9049","authenticated-orcid":false,"given":"Gaaminepreet","family":"Singh","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thakur Gurjeet","family":"Singh","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravinder Singh","family":"Jaura","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy Chitkara University  Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Goyal","sequence":"additional","affiliation":[{"name":"Government Medical College  Patiala Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/2231-0762.175416"},{"issue":"1279","key":"e_1_2_11_3_1","article-title":"Recent advances in understanding dengue","volume":"8","author":"Halstead S","year":"2019","journal-title":"F1000Research"},{"key":"e_1_2_11_4_1","first-page":"299","article-title":"Epidemiology of dengue past, present and future prospects","volume":"5","author":"Murray NE","year":"2013","journal-title":"Clin Epidemiol"},{"key":"e_1_2_11_5_1","unstructured":"World Health Organisation.\u00a02022. 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