{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:45:15Z","timestamp":1787319915853,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Serological markers for Epstein\u2013Barr virus (EBV) infection are commonly used to diagnose infectious mononucleosis and establish a serological status in pretransplant patients. This study prospectively assessed 1043 serum specimens sent to the laboratory for physician\u2010ordered EBV testing. The three markers\u2014antiviral capsid antigen (VCA) immunoglobulin M (IgM), anti\u2010VCA immunoglobulin G (IgG), and anti\u2010Epstein\u2013Barr nuclear antigen (EBNA) antibodies\u2014were tested using the Elecsys and the Liaison immunoassays. Specimens with discrepant results between the two assays were assessed using further EBV diagnostic approaches to conclude on the EBV serological status. In spite of substantial agreement between the two assays (88%) and with the presumed EBV status (&gt;92%), the results showed differences in the performance of the assays. Liaison VCA IgM appeared to be the most sensitive test for the detection of the 38 sera classified as early primary infection in comparison with the Elecsys assay (91.4% vs. 68.6%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.008). Excluding the cases of early primary infection, the sensitivity values of the VCA IgM marker were comparable between the Liaison and Elecsys assays (95.2% and 92.9%, respectively,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20091). Concerning the sera classified as past infection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009763), the Elecsys assay showed higher sensitivity values for the detection of the VCA and EBNA IgG markers in comparison with the Liaison assay (99.9% and 99.7% vs. 97.4% and 91.2%, respectively,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Overall, the Elecsys and Liaison assays showed similar performance. The interpretation of EBV serological profiles based on the clinical context may require serology follow up or further diagnostic approaches in challenging cases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.28166","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T22:58:00Z","timestamp":1663887480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of Elecsys and Liaison immunoassays to determine Epstein\u2013Barr virus serological status using further diagnostic approaches to clarify discrepant results"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6755-3115","authenticated-orcid":false,"given":"Julien","family":"Lupo","sequence":"first","affiliation":[{"name":"University Grenoble Alpes, Virology Laboratory Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"Institut de Biologie Structurale (IBS) University Grenoble Alpes, CEA, CNRS Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-6005","authenticated-orcid":false,"given":"Mansour","family":"Tsougaev","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes, Virology Laboratory Grenoble\u2010Alpes University Hospital Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5485-6918","authenticated-orcid":false,"given":"St\u00e9phane","family":"Blachier","sequence":"additional","affiliation":[{"name":"Laboratoire BioGroup Saint Martin d'H\u00e8res Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8823-1628","authenticated-orcid":false,"given":"Guillaume","family":"Chovelon","sequence":"additional","affiliation":[{"name":"Laboratoire BioGroup Saint Martin d'H\u00e8res Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7991-1733","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Truffot","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes, Virology Laboratory Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"Institut de Biologie Structurale (IBS) University Grenoble Alpes, CEA, CNRS Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0910-2209","authenticated-orcid":false,"given":"Corentin","family":"Leroy","sequence":"additional","affiliation":[{"name":"Clinical Investigation Center INSERM CIC1406 Grenoble\u2010Alpes University Hospital Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2424-8046","authenticated-orcid":false,"given":"Joris","family":"Giai","sequence":"additional","affiliation":[{"name":"Clinical Investigation Center INSERM CIC1406 Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"D\u00e9partement de M\u00e9thodologie et de l'Information de Sant\u00e9 Grenoble\u2010Alpes University Hospital Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8282-9522","authenticated-orcid":false,"given":"Olivier","family":"Epaulard","sequence":"additional","affiliation":[{"name":"Institut de Biologie Structurale (IBS) University Grenoble Alpes, CEA, CNRS Grenoble France"},{"name":"Infectious Diseases Unit Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"Groupe de recherche en infectiologie clinique, CIC\u20101406 Inserm\u2014CHUGA\u2014University Grenoble\u2010Alpes Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7600-5930","authenticated-orcid":false,"given":"Rapha\u00eble","family":"Germi","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes, Virology Laboratory Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"Institut de Biologie Structurale (IBS) University Grenoble Alpes, CEA, CNRS Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7456-0858","authenticated-orcid":false,"given":"Patrice","family":"Morand","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes, Virology Laboratory Grenoble\u2010Alpes University Hospital Grenoble France"},{"name":"Institut de Biologie Structurale (IBS) University Grenoble Alpes, CEA, CNRS Grenoble 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