{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:40:17Z","timestamp":1782888017810,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Monkeypox virus (MPXV) is a zoonotic orthopoxvirus within the Poxviridae family. MPXV is endemic to Central and West Africa. However, the world is currently witnessing an international outbreak with no clear epidemiological links to travel or animal exposure and with ever\u2010increasing numbers of reported cases worldwide.\u00a0Here, we evaluated and validated a new, sensitive, and specific real\u2010time PCR\u2010assay for MPXV diagnosis in humans and compare the performance of this novel assay against a Food &amp; Drug Administration\u2010cleared pan\u2010Orthopox RT\u2010PCR assay.\u00a0We determined specificity, sensitivity, and analytic performance of the PKamp\u2122 Monkeypox Virus RT\u2010PCR assay targeting the viral\n                      <jats:italic>F3L\u2010<\/jats:italic>\n                      gene. In addition, we further evaluated MPXV\u2010PCR\u2010positive specimens by viral culture, electron microscopy, and viral inactivation assays.\u00a0The limit of detection was established at 7.2 genome copies\/reaction, and MPXV was successfully identified in 20 clinical specimens with 100% correlation against the reference method with 100% sensitivity and specificity.\u00a0Our results demonstrated the validity of this rapid, robust, and reliable RT\u2010PCR assay for specific and accurate diagnosis of MPXV infection in human specimens collected both as dry swabs and in viral transport media. This assay has been approved by NYS Department of Health for clinical use.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.28247","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T00:18:58Z","timestamp":1666397938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Evaluation and validation of an RT\u2010PCR assay for specific detection of monkeypox virus (MPXV)"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1259-1736","authenticated-orcid":false,"given":"Alberto","family":"Paniz\u2010Mondolfi","sequence":"first","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York 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