{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:31:05Z","timestamp":1790051465062,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The current literature shows increasing concerns about potential seminal transmission of monkeypox virus (MPXV). Accordingly, we aimed to understand better the potential presence of MPXV in the seminal fluids and others specimens obtained from MPX cases.\u00a0On June 26, 2022, a systematic search of the literature was conducted to find articles that examine the presence of MPXV in the seminal fluid of confirmed cases. The search was updated once on August 12 and another on October 12, 2022, to include newly published articles. The prevalence of MPXV DNA presence in the seminal fluid and other specimens was pooled in a meta\u2010analysis (from studies with sample size\u2009&gt;\u20095 to reduce overestimation) and results were presented as effect sizes (ES) and their corresponding 95% confidence intervals (CI).\u00a0Nine articles were included. Only five studies were eligible for a meta\u2010analysis, and the pooled prevalence of MPXV DNA in semen specimens was 72.4% (95% CI: 55.7%\u221284.5%) among 115 patients. The positive rate of MPXV viral polymerase chain reaction\u00a0(PCR) was higher among skin samples (89%; 95%\u00a0CI: 78.2%\u221294.8%;\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200962; studies\u2009=\u20092), followed by anogenital\/rectal samples (74.3%; 95%\u00a0CI: 60.4%\u221284.5%;\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200954; studies\u2009=\u20092). On the other hand, the positivity rate was lower in nasopharyngeal (62.4%; 95%\u00a0CI: 20.4%\u221291.5%;\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u2009587; studies\u2009=\u20093), urine (21.1%; 95%\u00a0CI: 4.3%\u221261.1%;\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u2009617; studies\u2009=\u20094), and blood\/plasma (14.3%; 95%\u00a0CI: 11.3%\u221218.1%;\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u2009609; studies\u2009=\u20093) samples. Besides, MPXV can be detected in semen early from Day 1 and up to 19 days after symptoms onset. Finally, two articles investigated the infectivity of MPXV particles detected in seminal specimens by testing their replication competence. Culturing MPXV was successful in two out of four patients included in these studies.\u00a0MPXV is highly prevalent in seminal specimens of MPX cases, further corroborating the role of sexual transmission of the disease. However, further evidence is still needed to shed more light on the replication competence of these particles.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.28250","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T04:06:37Z","timestamp":1666411597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Monkeypox viral detection in semen specimens of confirmed cases: A systematic review and meta\u2010analysis"],"prefix":"10.1002","volume":"95","author":[{"given":"Abdullah","family":"Reda","sequence":"first","affiliation":[{"name":"Faculty of Medicine Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelaziz","family":"Abdelaal","sequence":"additional","affiliation":[{"name":"Tanta Research Team Tanta Egypt"},{"name":"Harvard Medical School Boston Massachusetts USA"},{"name":"Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aml M.","family":"Brakat","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Ash Sharqia Governorate Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basant Ismail","family":"Lashin","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Banha University Banha Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moustafa","family":"Abouelkheir","sequence":"additional","affiliation":[{"name":"Emergency Medicine Department, Pilgrim Hospital United Lincolnshire NHS Trust Boston UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2919-6196","authenticated-orcid":false,"given":"Basel","family":"Abdelazeem","sequence":"additional","affiliation":[{"name":"Tanta Research Team Tanta Egypt"},{"name":"McLaren Health Care Flint Michigan USA"},{"name":"Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso J.","family":"Rodriguez\u2010Morales","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Faculty of Medicine Fundacion Universitaria Autonoma de las Americas Pereira Risaralda Colombia"},{"name":"Institucion Universitaria Vision de las Americas  Pereira, Risaralda Colombia"},{"name":"Master of Clinical Epidemiology and Biostatistics Universidad Cientifica del Sur Lima Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjit","family":"Sah","sequence":"additional","affiliation":[{"name":"Harvard Medical School Boston Massachusetts USA"},{"name":"Tribhuvan University Teaching Hospital Institute of Medicine Kathmandu Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Centers for Disease Control and Prevention. Monkeypox: 2022 Global Map & Case Count. Accessed October 12 2022.https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world-map.html"},{"key":"e_1_2_9_3_1","volume-title":"Monkeypox, in StatPearls","author":"Moore MJ","year":"2022"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"13","article-title":"Human monkeypox","volume":"27","author":"Jezek Z","year":"1983","journal-title":"J Hyg Epidemiol Microbiol Immunol"},{"issue":"49","key":"e_1_2_9_5_1","first-page":"1168","article-title":"Human monkeypox\u2014Kasai Oriental, Democratic Republic of Congo, February 1996\u2010October 1997","volume":"46","author":"Centers for Disease Control and Prevention (CDC)","year":"1997","journal-title":"MMWR Morb Mortal Wkly 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