{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:54:55Z","timestamp":1787849695899,"version":"build-2784847793"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The ongoing outbreak of monkeypox virus (MPXV) is the largest one in historically non\u2010endemic countries. Early reports described atypical epidemiological and clinical presentations. We investigated MPXV DNA detection in oropharyngeal samples (OPS), and compared the viral load to that in lesion samples at diagnosis in patients infected with MPXV. We retrospectively included patients suspected to have monkeypox in Northern France, who underwent a MPXV PCR in the Virology Laboratory, University Hospital of Lille, from May 23\u00a0to August 18, 2022. Overall, a total of 228 patients (376 samples) were included. A positive result in at least one sample was found in 138 patients (60.5%). We compared PCR results between OPS\u00a0and lesion samples (i.e., cutaneous or anal\/rectal samples) in patients with both samples. A positive result in OPS was observed in 54 out of 60 patients (90%). The viral load in OPS (median\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>t<\/jats:sub>\n                    value\u2009=\u200929.5; interquartile range\u00a0[IQR]\u2009=\u200924.7\u221234) was significantly lower than that in lesion samples (median\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>t<\/jats:sub>\n                    \u00a0value\u2009=\u200917.8; IQR\u2009=\u200916.3 and 19.7) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). This report shows that pharyngeal sampling does not bring additional information for the initial diagnosis in patients presenting with typical lesions.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28276","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T06:03:47Z","timestamp":1667455427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Oropharyngeal samples versus lesion specimens at diagnosis in patients infected with monkeypox virus in Northern France"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6544-9795","authenticated-orcid":false,"given":"Mahdi","family":"Ouafi","sequence":"first","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-7772","authenticated-orcid":false,"given":"Alexandre","family":"Regueme","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Alcaraz","sequence":"additional","affiliation":[{"name":"CH Tourcoing Service Universitaire de Maladies Infectieuses et Tropicales Tourcoing France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Riviere","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Maladies Infectieuses CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hel\u00e8ne","family":"Bazus","sequence":"additional","affiliation":[{"name":"CH Lens Service de Maladies Infectieuses et Tropicales Lens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Salmon\u2010Rousseau","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Maladies Infectieuses CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Cappeliez","sequence":"additional","affiliation":[{"name":"CH Tourcoing Service Universitaire de Maladies Infectieuses et Tropicales Tourcoing France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mie","family":"Cartier","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Guigon","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouna","family":"Lazrek","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Bocket","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Faure","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Maladies Infectieuses CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Robineau","sequence":"additional","affiliation":[{"name":"CH Tourcoing Service Universitaire de Maladies Infectieuses et Tropicales Tourcoing France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Hober","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enagnon Kazali","family":"Alidjinou","sequence":"additional","affiliation":[{"name":"Univ Lille, CHU Lille Laboratoire de Virologie ULR3610 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.22.2200422"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.24.2200482"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.12513"},{"key":"e_1_2_9_5_1","unstructured":"CDC. Monkeypox outbreak global map.\u00a02022. Accessed August 19 2022.\u00a0https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world-map.html"},{"key":"e_1_2_9_6_1","unstructured":"ECDC. Monkeypox multi\u2010country outbreak.\u00a02022. Accessed August 19 2022.\u00a0https:\/\/www.ecdc.europa.eu\/en\/monkeypox-outbreak"},{"key":"e_1_2_9_7_1","unstructured":"WHO. Monkeypox.\u00a02022. Accessed August 16 2022.https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/monkeypox"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00513-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.14857"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61602-X"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001885"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00411-X"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2207323"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-072410"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01436-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00228-6"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28276","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.28276","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:43:52Z","timestamp":1772959432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,9]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/jmv.28276"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28276","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,9]]},"assertion":[{"value":"2022-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28276"}}