{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:09:24Z","timestamp":1772982564202,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Respiratory syncytial virus (RSV) is the most important virus that causes lower respiratory tract disease in children; efficient viral identification is an important component of disease prevention and treatment. Here, we developed and evaluated a ready\u2010to\u2010use (RTU) nucleic acid extraction\u2010free direct reagent for identification of RSV (RTU\u2010Direct test) in clinical samples. The limit of detection (LOD) of the RSV RTU\u2010Direct test was consistent with the LOD of the standard test using extracted nucleic acids. The virus inactivation ability of RTU\u2010Direct reagent was confirmed by viral infectivity assays involving RTU\u2010Direct\u2010treated samples containing RSV and human coronavirus OC43. RSV RNA stability was significantly better in RTU\u2010Direct reagent than in conventional virus transport medium (VTM) at room temperature and 4\u00b0C (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The clinical performance of the RTU\u2010Direct test was evaluated using 155 respiratory specimens from patients with suspected RSV infection. Positive agreement between the RTU\u2010Direct test and the VTM standard test was 100% (42\/42); negative agreement was 99.1% (112\/113), and the kappa statistic was 0.968 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). The distributions of Ct values did not significantly differ between the RTU\u2010Direct test and the standard test (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Overall, the RTU\u2010Direct reagent can improve the efficiency and biosafety of RSV detection, while reducing the cost of detection.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28287","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T02:18:57Z","timestamp":1667873937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improving the efficiency and biosafety of respiratory syncytial virus identification using a nucleic acid extraction\u2010free reagent"],"prefix":"10.1002","volume":"95","author":[{"given":"Shuyan","family":"Qiu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duo","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Dai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingui","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou 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Guangzhou Institute of Respiratory Health, Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Disease Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00037-07"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60206-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-013-8368-9"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03693-x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165108"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096674"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prrv.2018.12.001"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.284921"},{"issue":"1","key":"e_1_2_11_10_1","first-page":"95","article-title":"The effectiveness of ribavirin in the treatment of 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