{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:08:57Z","timestamp":1772982537653,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Monkeypox virus (MPXV) is a member of\n                    <jats:italic>Orthopoxvirus<\/jats:italic>\n                    in the Poxviridae family, causing a Public Health Emergency of International Concern. The number of cases and geographic range has increased significantly in 2022. Identification of MPXV\u2010specific therapeutic targets is urgent. G\u2010quadruplex (GQ) secondary structures attract great attention as potential targets for antiviral strategy. Whether GQs are present in the\u00a0MPXV genome remains inconclusive. In this study, we aim to characterize the GQs encoded by MPXV. Through a series of biophysical experiments, we characterized the formation potential of MPXV\u2010encoded GQs and evaluated the binding and stabilization abilities of GQ ligands including BRACO\u201019, pyridostatin, and TMPyP4 to GQs encoded by MPXV. Moreover, GQ ligands suppressed the gene transcription of MPXV sequences containing GQ. BRACO\u201019 and TMPyP4 were able to inhibit vaccinia virus replication. We demonstrated the existence of MPXV GQ and reinforced the idea that GQs could be novel antiviral targets. Targeting these GQ sequences with GQ\u2010binding molecules may represent a new approach for MPXV therapy.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28299","type":"journal-article","created":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T03:54:58Z","timestamp":1668138898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["G\u2010quadruplexes in the monkeypox virus are potential antiviral targets"],"prefix":"10.1002","volume":"95","author":[{"given":"Lu","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"Department of Pathogen Biology, School of Clinical and Basic Medical Sciences Shandong First Medical University and Shandong Academy of Medical Sciences  Jinan Shandong China"},{"name":"Institute of Infection and Immunity, Medical Science and Technology Innovation Center Shandong First Medical University and Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7015-9661","authenticated-orcid":false,"given":"Leiliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"Department of Pathogen Biology, School of Clinical and Basic Medical Sciences Shandong First Medical University and Shandong Academy of Medical Sciences  Jinan Shandong China"},{"name":"Institute of Infection and Immunity, Medical Science and Technology Innovation Center Shandong First Medical University and Shandong Academy of Medical Sciences  Jinan Shandong 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