{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:42:19Z","timestamp":1788280939043,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Community\u2010acquired pneumonia (CAP) is a serious clinical concern. A lack of accurate diagnosis could hinder pathogen\u2010directed therapeutic strategies. To solve this problem, we evaluated clinical application of nested multiplex polymerase chain reaction (PCR) in children with severe CAP. We prospectively enrolled 60 children with severe CAP requiring intensive care between December 2019 and November 2021 at a tertiary medical center. Nested multiplex PCR respiratory panel (RP) and pneumonia panel (PP) were performed on upper and lower respiratory tract specimens. We integrated standard\u2010of\u2010care tests and quantitative PCR for validation. The combination of RP, PP, and standard\u2010of\u2010care tests could detect at least one pathogen in 98% of cases and the mixed viral\u2010bacterial detection rate was 65%. The positive percent agreement (PPA), and negative percent agreement (NPA) for RP were 94% and 99%; the PPA and NPA for PP were 89% and 98%. The distribution of pathogens was similar in the upper and lower respiratory tracts, and the DNA or RNA copies of pathogens in the lower respiratory tract were equal to or higher than those in the upper respiratory tract. PP detected bacterial pathogens in 40 (67%) cases, and clinicians tended to increase bacterial diagnosis and escalate antimicrobial therapy for them.\u00a0RP and PP had satisfactory performance to help pediatricians make pathogenic diagnoses and establish therapy earlier. The pathogens in the upper respiratory tract had predictive diagnostic values for lower respiratory tract infections in children with severe CAP.<\/jats:p>","DOI":"10.1002\/jmv.28334","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T07:36:47Z","timestamp":1669275407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Application of nested multiplex polymerase chain reaction respiratory and pneumonia panels in children with severe community\u2010acquired pneumonia"],"prefix":"10.1002","volume":"95","author":[{"given":"Ting\u2010Yu","family":"Yen","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University Taipei Taiwan"},{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Fu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"En\u2010Ting","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Chia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank Leigh","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Min","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University Taipei Taiwan"},{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2632-1956","authenticated-orcid":false,"given":"Luan\u2010Yin","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College 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