{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:03:08Z","timestamp":1789909388476,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) has caused a global coronavirus disease 2019 (COVID\u201019) pandemic that has affected the lives of billions of individuals. However, the host\u2010virus interactions still need further investigation to reveal the underling mechanism of SARS\u2010CoV\u20102 pathogenesis. Here, transcriptomics analysis of SARS\u2010CoV\u20102 infection highlighted possible correlation between host\u2010associated signaling pathway and virus. In detail, cAMP\u2010protein kinase (PKA) pathway has an essential role in SARS\u2010CoV\u20102 infection, followed by the interaction between cyclic AMP response element binding protein (CREB) and CREB\u2010binding protein (CBP) could be induced and leading to the enhancement of CREB\/CBP transcriptional activity. The replication of Delta and Omicron BA.5 were inhibited by about 49.4% and 44.7% after knockdown of CREB and CBP with small interfering RNAs, respectively. Furthermore, a small organic molecule naphthol AS\u2010E (nAS\u2010E), which targets on the interaction between CREB and CBP, potently inhibited SARS\u2010CoV\u20102 wild\u2010type (WT) infection with comparable the half\u2010maximal effective concentration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ) 1.04\u2009\u03bcM to Remdesivir 0.57\u2009\u03bcM. Compared with WT virus, EC\n                    <jats:sub>50<\/jats:sub>\n                    in Calu\u20103 cells against Delta, Omicron BA.2, and Omicron BA.5 were, on average, 1.5\u2010fold, 1.1\u2010fold, and 1.5\u2010fold higher, respectively, nAS\u2010E had a satisfied antiviral effect against Omicron variants. Taken together, our study demonstrated the importance of CREB\/CBP induced by cAMP\u2010PKA pathway during SARS\u2010CoV\u20102 infection, and further provided a novel CREB\/CBP interaction therapeutic drug targets for COVID\u201019.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28383","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T09:23:31Z","timestamp":1670491411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["SARS\u2010CoV\u20102 infection activates CREB\/CBP in cellular cyclic AMP\u2010dependent pathways"],"prefix":"10.1002","volume":"95","author":[{"given":"Qi","family":"Yang","sequence":"first","affiliation":[{"name":"Chen Xinwen Lab in Department of Basic Research Guangzhou Laboratory  Guangzhou China"},{"name":"Hepatitis Virus and Gene Therapy Lab, State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega\u2010Science Chinese Academy of Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jielin","family":"Tang","sequence":"additional","affiliation":[{"name":"Chen Xinwen Lab in Department of Basic Research Guangzhou Laboratory  Guangzhou China"},{"name":"Hepatitis Virus and Gene Therapy Lab, State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega\u2010Science Chinese Academy of Sciences  Wuhan China"},{"name":"Center for Infection &amp; Immunity, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Cao","sequence":"additional","affiliation":[{"name":"Hepatitis Virus and Gene Therapy Lab, State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega\u2010Science Chinese Academy of Sciences  Wuhan 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