{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T20:46:14Z","timestamp":1789073174555,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The global outbreak of the monkeypox virus (MPXV) highlights the need for rapid and cost\u2010effective MPXV detection tools to effectively monitor and control the monkeypox disease. Herein, we demonstrated a portable CRISPR\u2013Cas\u2010based system for naked\u2010eye detection of MPXV. The system harnesses the high selectivity of CRISPR\u2013Cas12 and the isothermal nucleic acid amplification potential of recombinase polymerase amplification. It can detect both the current circulating MPXV clade and the original clades. We reached a\u00a0limit of detection (LoD) of 22.4\u2009aM (13.5\u2009copies\/\u00b5l) using a microtiter plate reader, while the visual LoD of the system is 75\u2009aM (45\u2009copies\/\u00b5l) in a two\u2010step assay, which is further reduced to 25\u2009aM (15\u2009copies\/\u00b5l) in a one\u2010pot system. We compared our results with quantitative polymerase chain reaction and\u00a0obtained satisfactory consistency. For clinical application, we demonstrated a sensitive and precise visual detection method with attomolar sensitivity and a sample\u2010to\u2010answer time of 35\u2009min.<\/jats:p>","DOI":"10.1002\/jmv.28385","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T09:28:11Z","timestamp":1670491691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["CRISPR\u2013Cas12\u2010based field\u2010deployable system for rapid detection of synthetic DNA sequence of the monkeypox virus genome"],"prefix":"10.1002","volume":"95","author":[{"given":"Qun","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School Tsinghua University  Shenzhen P. R. China"},{"name":"Tsinghua Shenzhen International Graduate School,\u00a0Tsinghua\u2010Berkeley Shenzhen Institute Tsinghua University  Shenzhen P. R. 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