{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:06:45Z","timestamp":1772982405258,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Epstein\u2013Barr virus (EBV)\u2010associated gastric cancer (EBVaGC) is a distinct subtype of gastric cancer (GC) distinguished by the presence of the EBV genome and limited viral gene expression within malignant epithelial cells. EBV infection is generally thought to be a relatively late event following atrophic gastritis in carcinogenesis, which implies the heterogeneity of EBVaGC. To facilitate the study of the role of EBV in EBVaGC, we established two EBV\u2010positive GC cell lines (AGS\u2010EBV and HGC27\u2010EBV) with an epitheliotropic EBV strain M81 and characterized viral and cellular gene expression profiles in comparison to SNU719, a naturally derived EBV\u2010positive GC cell line. Like SNU719, AGS\u2010EBV and HGC27\u2010EBV stably maintained their EBV genomes and expressed EBV\u2010encoded small RNAs and nuclear antigen EBNA1. Comprehensive analysis of the expression of EBV\u2010encoded miRNAs within the\n                    <jats:italic>Bam<\/jats:italic>\n                    HI\u2010A region rightward transcript region, and the transcripts of EBV latent and lytic genes in cell lines, as well as xenografts, reveals that AGS\u2010EBV and HGC27\u2010EBV cells undergo distinct viral expression profiles. A very small fraction of AGS\u2010EBV and SNU719 cells can spontaneously produce infectious progeny virions, while HGC27\u2010EBV does not. AGS\u2010EBV (both M81 and Akata) cells largely mimic SNU719 cells in viral gene expression profiles, and altered cellular functions and pathways perturbed by EBV infection. Phylogenetic analysis of the EBV genome shows both M81 and Akata EBV strains are closely related to clinical EBVaGC isolates. Taken together, these two newly established EBV\u2010positive GC cell lines can serve as models to further investigate the role of EBV in different contexts of gastric carcinogenesis and identify novel therapeutics against EBVaGC.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28387","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T09:23:30Z","timestamp":1670491410000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Establishment and characterization of two Epstein\u2013Barr virus\u2010positive gastric cancer cell lines with epitheliotropic M81 strain undergoing distinct viral and altered cellular expression profiles"],"prefix":"10.1002","volume":"95","author":[{"given":"Mingqian","family":"Xu","sequence":"first","affiliation":[{"name":"Laboratory of Human Virology and Oncology Shantou University Medical College Shantou Guangdong 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