{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:04:31Z","timestamp":1788887071864,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cervical cancer prevention is based on primary prevention with vaccines against Human Papillomavirus (HPV) and secondary prevention by screening with High\u2010Risk\u2010HPV (Hr\u2010HPV) detection. Since 2017, cervical cancer screening in women aged 25\u221260 years has been performed in Portugal using Hr\u2010HPV detection, followed by cytology in Hr\u2010HPV\u2010positive cases.\u00a0Herein we report the prevalence of Hr\u2010HPV genotypes and cytological abnormalities among 462\u2009401 women (mean age: 43.73\u2009\u00b1\u200910.79; median age: 45; range: 24\u221266 years) that participated in the Regional Cervical Cancer Screening Program of the Northern Region of Portugal, performed between August 2016 and December 2021.\u00a0Overall, we describe a prevalence rate of 12.50% for Hr\u2010HPV varying from 20.76% at age 25% to 8.32% at age 64. The five most common Hr\u2010HPV genotypes identified were HPV\u201068 (16.09%), HPV\u201031 (15.30%), HPV\u201051 (12.96%), HPV\u201016 (11.06%), and HPV\u201039 (11.01%). The prevalence of Hr\u2010HPV included in the nonavalent vaccine (HPV\u20109valent) was 55.00% ranging from 47.78% to 59.18% across different age groups. Considering positive Hr\u2010HPV cases, 65.68% had a Negative for Intraepithelial Lesion or Malignancy (NILM) cytology, 20.83% atypical squamous cells of undetermined significance (ASC\u2010US), 8.85% Low\u2010Grade Squamous Intraepithelial Lesion (LSIL), 1.65% High\u2010Grade Squamous Intraepithelial Lesion (HSIL), 2.85% ASC\u2010H, 0.09% Atypical Glandular Cells, 0.02% Adenocarcinomas, and 0.02% Squamous Cell Carcinoma (SCC). Our analysis revealed that HPV\u20109val genotypes were responsible for 52.13% NILM, 59.21% ASC\u2010US, 55.06% LSIL, 90.14% HSIL, 83.50% ASC\u2010H, and 100.00% SCC. Furthermore, multiple Hr\u2010HPV infections (risk ratio\u00a0[RR]\u2009=\u20091.46; 95% confidence interval [CI] 1.34\u22121.58), HPV\u201016\/18 (RR\u2009=\u20095.16; 95% CI 4.75\u22125.93), or HPV\u20109val genotypes (RR\u2009=\u20095.23; 95% CI 4.68\u22125.85) were associated with a significant risk of developing\u2009&gt; \u2009HSIL (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001).\u00a0To date, this is the largest study on Hr\u2010HPV genotyping in cervical cancer screening that includes data from a complete cycle of the screening program. Our findings suggest a high prevalence of HPV\u20109valent genotypes and a significant association with an increased risk of developing\u2009&gt;\u2009HSIL. This constitutes important data for health authorities, which may help define the future of vaccination and cervical cancer screening strategies.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28414","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T08:48:00Z","timestamp":1671612480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Impact of high\u2010risk Human Papillomavirus genotyping in cervical disease in the Northern region of Portugal: Real\u2010world data from regional cervical cancer screening program"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4348-0596","authenticated-orcid":false,"given":"Andreia","family":"Ros\u00e1rio","sequence":"first","affiliation":[{"name":"Molecular Oncology &amp; Viral Pathology Group, Research Center (CI\u2010IPOP)\/RISE@CI\u2010IPOP (Health Research Network) Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"},{"name":"Department of Pathology and Laboratory Medicine, Clinical Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3128-3328","authenticated-orcid":false,"given":"Ana","family":"Sousa","sequence":"additional","affiliation":[{"name":"Molecular Oncology &amp; Viral Pathology Group, Research Center (CI\u2010IPOP)\/RISE@CI\u2010IPOP (Health Research Network) Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"},{"name":"Department of Health Sciences Escola Superior de Sa\u00fade do Instituto Polit\u00e9cnico de Bragan\u00e7a  Bragan\u00e7a Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2165-1163","authenticated-orcid":false,"given":"Joana","family":"Marinho\u2010Dias","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Clinical Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-8373","authenticated-orcid":false,"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Molecular Oncology &amp; Viral Pathology Group, Research Center (CI\u2010IPOP)\/RISE@CI\u2010IPOP (Health Research Network) Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"},{"name":"Department of Pathology and Laboratory Medicine, Clinical Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"},{"name":"Research Department Portuguese League Against Cancer (LPCC\u2010NRNorte)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Anatomic Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"Le\u00e7a","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Anatomic Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7809-6597","authenticated-orcid":false,"given":"Nuno","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Anatomic Pathology Service Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Tavares","sequence":"additional","affiliation":[{"name":"Administra\u00e7\u00e3o Regional de Sa\u00fade do Norte I.P.  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