{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:34:25Z","timestamp":1789029265953,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The coronavirus disease 2019 (COVID\u201019) pandemic has emphasized the need for development of a rapid diagnostic device for the effective treatment and its mitigation. Lateral flow immunoassay (LFIA) belongs to a class of diagnostic devices, which has the benefit of providing quick results, easy to handle, low cost, and on\u2010site applicable. So far, several LFIA has been developed for the detection of infectious severe acute respiratory syndrome\u2010coronavirus\u20102\u00a0(SARS\u2010CoV\u20102), however, only a few of them are antigen (Ag)\u2010based. Here, we describe an antibody (Ab)\u2010labeled gold nanoparticles (AuNPs)\u2010based LFIA\u00a0(AuNPs\u2010LFIA) for the detection of Receptor\u2010Binding Domain (RBD) of SARS\u2010CoV\u20102. For this, RBD Ab\u00a0of SARS\u2010CoV\u20102 was conjugated with the AuNPs, which served as a detecting probe. The fabricated LFIA strip was optimized for different parameters such as membrane pore size, blocking conditions, Ab coating concentration, and conjugate incubation. The optimized LFIA strips were validated in spiked buffer samples and the optimal limit of detection was found to be 1\u2009ng\/ml, which was confirmed by a smartphone\u2010based application. Moreover, the developed AuNPs\u2010LFIA strips effectively detected RBD Ag\u00a0in 100 clinical samples with 94.3% sensitivity and 90.9% specificity\u00a0in clinical samples when compared with the gold standard (RT\u2010PCR). The fabricated LFIAs are reported to have storage stability of up to 21 days at 4\u00b0C and room temperature (RT).\u00a0Hence, the developed LFIA can be used as a portable, cost\u2010effective diagnostic device for rapid detection of SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/jmv.28416","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T08:47:49Z","timestamp":1671612469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Gold nanoparticle conjugate\u2010based lateral flow immunoassay (LFIA) for rapid detection of RBD antigen of SARS\u2010CoV\u20102 in clinical samples using a smartphone\u2010based application"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0856-245X","authenticated-orcid":false,"given":"Drishya","family":"Prakashan","sequence":"first","affiliation":[{"name":"DBT\u2010National Institute of Animal Biotechnology (NIAB)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narlawar S.","family":"Shrikrishna","sequence":"additional","affiliation":[{"name":"DBT\u2010National Institute of Animal Biotechnology (NIAB)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Byakodi","sequence":"additional","affiliation":[{"name":"DBT\u2010National Institute of Animal Biotechnology (NIAB)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Nagamani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gandhi Medical College Gandhi Hospital  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0960-2780","authenticated-orcid":false,"given":"Sonu","family":"Gandhi","sequence":"additional","affiliation":[{"name":"DBT\u2010National Institute of Animal Biotechnology (NIAB)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.31219\/osf.io\/6eagn"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2798-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1719902"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0242128"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.605702"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105951"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01909-w"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2020.09.019"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.725240"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.2c00503"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c06392"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","unstructured":"XiangJ YanM LiH et al. 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