{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:29:35Z","timestamp":1788380975593,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Development of biomarkers for predicting the occurrence of hepatitis E virus related\u2010acute liver failure (HEV\u2010ALF) is conducive to prevention and early intervention. Serum samples from 250 HEV\u2010ALF patients, 250 patients with acute hepatitis E (AHE) and 250 health controls (HCs) were collected. We assessed the predictive ability of extracellular vesicle (EV)\u2010derived argininosuccinate synthase 1 (ASS1) levels for HEV\u2010ALF occurrence. Serum EVs were successfully isolated. EV\u2010derived ASS1 levels in the HEV\u2010ALF patients were significantly higher than those in the AHE patients and HCs. In HEV\u2010ALF patients, EV\u2010derived ASS1 levels were positively correlated with the number of failed organs and disease progression. The logistical regression showed that EV\u2010derived ASS1 level is an independent risk factor for HEV\u2010ALF, and orthogonal partial least squares discriminant analysis (OPLS\u2010DA) also suggested that EV\u2010derived ASS1 level has high predictive capability. Besides, the area under the curve (AUC) of EV\u2010derived ASS1 level to predict HEV\u2010ALF occurrence was 0.728 (0.684\u20130.772) with the sensitivity and specificity being 72.80% and 64.80%, which had a high decision\u2010making ability. Furthermore, there existed no significant difference between the age \u226560 and age &lt;60 groups in EV\u2010derived ASS1 levels. Serum EV\u2010derived ASS1 level is a promising predictor for the occurrence of HEV\u2010ALF.<\/jats:p>","DOI":"10.1002\/jmv.28425","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T06:05:26Z","timestamp":1671775526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Serum extracellular vesicle\u2010derived ASS1 is a promising predictor for the occurrence of HEV\u2010ALF"],"prefix":"10.1002","volume":"95","author":[{"given":"Ze","family":"Xiang","sequence":"first","affiliation":[{"name":"Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School Nanjing Medical University  Suzhou Jiangsu 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Medicine Xuzhou Medical University Xuzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Yancheng Clinical College of Xuzhou Medical University The First people's Hospital of Yancheng Yancheng Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infection Management, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School Nanjing Medical University Suzhou Jiangsu 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