{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:07:12Z","timestamp":1773248832716,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T00:00:00Z","timestamp":1672444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T00:00:00Z","timestamp":1672444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The immune response is crucial for coronavirus disease 19 (COVID\u201019) progression, with the participation of proinflammatory cells and cytokines, inducing lung injury and loss of respiratory function. CLEC5A expression on monocytes can be triggered by viral and bacterial infections, leading to poor outcomes. Severe acute respiratory syndrome coronavirus 2\u00a0(SARS\u2010CoV\u20102) is able to induce neutrophil activation by CLEC5A and Toll\u2010like receptor 2, leading to an aggressive inflammatory cascade, but little is known about the molecular interactions between CLEC5A and SARS\u2010CoV\u20102 proteins. Here, we aimed to explore how CLEC5A expression could be affected by SARS\u2010CoV\u20102 infection using immunological tools with in vitro, in vivo, and in silico assays. The findings revealed that high levels of CLEC5A expression were found in monocytes from severe COVID\u201019 patients in comparison with mild COVID\u201019 and unexposed subjects, but not in vaccinated subjects who developed mild COVID\u201019. In hamsters, we detected\n                    <jats:italic>CLEC5A<\/jats:italic>\n                    gene expression during 3\u201315 days of Omicron strain viral challenge. Our results also showed that CLEC5A can interact with SARS\u2010CoV\u20102, promoting inflammatory cytokine production, probably through an interaction with the receptor\u2010binding domain in the N\u2010acetylglucosamine binding site (NAG\u2010601). The high expression of CLEC5A and high levels of proinflammatory cytokine production were reduced in vitro by a human CLEC5A monoclonal antibody. Finally, CLEC5A was triggered by spike glycoprotein, suggesting its involvement in COVID\u201019 progression; therapy with a monoclonal antibody could be a good strategy for COVID\u201019 treatment, but vaccines are still the best option to avoid hospitalization\/deaths.\n                  <\/jats:p>","DOI":"10.1002\/jmv.28427","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T06:28:38Z","timestamp":1672036118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["CLEC5A expression can be triggered by spike glycoprotein and may be a potential target for COVID\u201019 therapy"],"prefix":"10.1002","volume":"95","author":[{"given":"Thiago L.","family":"Machado","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alanna C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hepatites Virais, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamiris","family":"Azamor","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea M. V.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa R.","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana N.","family":"Tubar\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre dos Santos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento Tecnol\u00f3gico em Virologia, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Del Ros\u00e1rio","family":"Flores Rodrigues","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento Tecnol\u00f3gico em Virologia, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio\u2010Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo A.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento Tecnol\u00f3gico em Virologia, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-8969","authenticated-orcid":false,"given":"Livia M.","family":"Villar","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hepatites Virais, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana P. 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