{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:50:16Z","timestamp":1792648216214,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,15]],"date-time":"2023-01-15T00:00:00Z","timestamp":1673740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["Z211100002121042"],"award-info":[{"award-number":["Z211100002121042"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle\u2010based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV\u2010MCDA\u2010LFB) and differentiation of West and Central African MPXV isolates. The MPXV\u2010MCDA\u2010LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64\u00b0C for 30\u2009min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV\u2010MCDA\u2010LFB assay could be completed within 50\u2009min including rapid template preparation (15\u2009min), MCDA reaction (30\u2009min) and reporting of result (&lt;5\u2009min). The MPXV\u2010MCDA\u2010LFB method is very sensitive and can detect the target genes (D14L and ATI) with as low as five copies of plasmid template per reaction and 12.5 copies of pseudotyped virus in human blood samples. The MPXV\u2010MCDA\u2010LFB assay does not cross\u2010react with non\u2010MPXV templates, validating its specificity. Therefore, the MPXV\u2010MCDA\u2010LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.<\/jats:p>","DOI":"10.1002\/jmv.28479","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T08:14:35Z","timestamp":1673079275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle\u2010based biosensor platform"],"prefix":"10.1002","volume":"95","author":[{"given":"Juan","family":"Zhou","sequence":"first","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Fu","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Jia","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Huang","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunrong","family":"Sun","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Huan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Affiliated Children's Hospital Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2031-087X","authenticated-orcid":false,"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Experimental Research Center Capital Institute of Pediatrics Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2022.2088881"},{"key":"e_1_2_9_3_1","first-page":"165","article-title":"Human monkeypox, 1970\u201079","volume":"58","author":"Breman JG","year":"1980","journal-title":"Bull World Health Organ"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a011720"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0703.017311"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032299"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2505.190076"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.38.1800509"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30537-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lana.2022.100318"},{"key":"e_1_2_9_11_1","volume-title":"Emergency situational updates; 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