{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:13:52Z","timestamp":1790882032040,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,5]],"date-time":"2023-02-05T00:00:00Z","timestamp":1675555200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In early May 2022, the first worldwide monkeypox virus (MPXV) outbreak was reported, with different clinical aspects from previously studied human monkeypox infections. Despite monkeypox medical importance, much of its biological aspects remain to be further investigated. In the present work, we evaluated ultrastructural aspects of MPXV\u00a0asynchronous infections in Vero cells by transmission electron microscopy (TEM). The viral strain was isolated from a male patient infected during the 2022 outbreak. TEM analysis showed: (i) adhered intracellular mature virus particles before entry of the host cell; (ii) a reorganization of the rough endoplasmic reticulum cisternae into the so\u2010called \u201cmini\u2010nuclei\u201d\u00a0structure associated with genome replication; and (iii) noticeably different sites within the viral factory presenting granular or fibrillar aspects. We also observed viral crescents, different MPXV\u00a0particle morphotypes, and cellular alterations induced by infection, such as changes in the cytoskeleton structure and multimembrane vesicles abundance. Taken together, to the best of our knowledge, these results revealed for the first\u2010time ultrastructural aspects of different steps of the MPXV\u00a0cycle.<\/jats:p>","DOI":"10.1002\/jmv.28536","type":"journal-article","created":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T07:44:40Z","timestamp":1674891880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Ultrastructural analysis of monkeypox virus replication in Vero cells"],"prefix":"10.1002","volume":"95","author":[{"given":"Amanda St\u00e9phanie Arantes","family":"Witt","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giliane de Souza","family":"Trindade","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Gil de","family":"Souza","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus S\u00e1 Magalh\u00e3es","family":"Serafim","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alana Vitor Barbosa","family":"da Costa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias, Diretoria do Instituto Oct\u00e1vio Magalh\u00e3es, Servi\u00e7o de Virologia e Riquetsioses  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Vin\u00edcius Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias, Diretoria do Instituto Oct\u00e1vio Magalh\u00e3es, Servi\u00e7o de Virologia e Riquetsioses  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Campos","family":"de Melo Iani","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias, Diretoria do Instituto Oct\u00e1vio Magalh\u00e3es, Servi\u00e7o de Virologia e Riquetsioses  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Ara\u00fajo Lima","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erna Geessien","family":"Kroon","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9420-1791","authenticated-orcid":false,"given":"J\u00f4natas Santos","family":"Abrah\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1959.tb00328.x"},{"issue":"5","key":"e_1_2_10_3_1","first-page":"593","article-title":"A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo","volume":"46","author":"Ladnyj ID","year":"1972","journal-title":"Bull World Health Organ"},{"issue":"5","key":"e_1_2_10_4_1","first-page":"599","article-title":"Isolation and properties of the causal agent of a new variola\u2010like disease (monkeypox) in man","volume":"46","author":"Marennikova SS","year":"1972","journal-title":"Bull World Health Organ"},{"key":"e_1_2_10_5_1","unstructured":"Centers for Disease Control and Prevention. 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