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While several studies have suggested the efficacy of ribavirin against CCHFV, current literature remains inconclusive. In this study, we have utilised next\u2010generation sequencing to investigate the mutagenic effect of ribavirin on the CCHFV genome during clinical disease. Samples collected from CCHF patients receiving ribavirin treatment or supportive care only at Sivas Cumhuriyet University Hospital, Turkey, were analysed. By comparing the frequency of mutations in each group, we found little evidence of an overall mutagenic effect. This suggests that ribavirin, administered at the acute stages of CCHFV infection (at the World Health Organization\u2010recommended\u00a0dose) is unable to induce lethal mutagenesis that would cause an extinction event in the CCHFV population and reduce viremia.<\/jats:p>","DOI":"10.1002\/jmv.28548","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T08:07:04Z","timestamp":1675411624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Investigating the effect of ribavirin treatment on genetic mutations in Crimean\u2013Congo haemorrhagic fever virus (CCHFV) through next\u2010generation sequencing"],"prefix":"10.1002","volume":"95","author":[{"given":"Jake","family":"D'Addiego","sequence":"first","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"},{"name":"Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazif","family":"Elaldi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology Sivas Cumhuriyet University Faculty of Medicine  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadina","family":"Wand","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Osman","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binnur Koksal","family":"Bagci","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Faculty of Health Sciences Sivas Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Kennedy","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse Nur","family":"Pektas","sequence":"additional","affiliation":[{"name":"Cumhuriyet University Advanced Technology Application and Research Center (CUTAM) Sivas Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eilish","family":"Hart","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Slack","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Hewson","sequence":"additional","affiliation":[{"name":"UK Health Security Agency  Salisbury UK"},{"name":"Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"World Health Organization. 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