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Our study aims to investigate the genomic diversity of the CCHF virus circulating in Punjab and Khyber Pakhtunkhwa provinces of Pakistan. Between April to September 2022, 46 samples from suspected CCHF patients were tested, with 6\u00a0(13%) showing positive RT\u2010PCR results. Among the positive cases, all were male, aged 14\u221248 years among which 4\u00a0were butchers. Three CCHF patients succumbed to the disease. The complete S\u2010M\u2010L\u2010gene\u00a0fragments of 4\u00a0positive samples were sequenced. The S and L segments belonged to the Asia\u20101 clade and clustered with regional strains from Iran, India, and Afghanistan. One M segment sequence grouped into Africa\u20102 along with those reported from India during 2016\u22122019. We report the detection of a reassorted virus (NIH\u2010PAK\u2010CCHF\u201003|2022) having Asia\u20101\u2010Africa\u20102\u2010Asia\u20101 (S\u2010M\u2010L) segment pattern for the first time from Pakistan. Mutational analysis showed M segments harboring eight mutations (T55A, S80P, T110I, T185A, T189A, A212T, and N239I\/T) in the mucin\u2010like domain, five mutations (D250N, T333S, I375V, M401I, A433T), four mutations (N545D, Y657F, K688R, and I824V) in GP38\u2010domain, and three mutations (T1418N, A1431V, and G1449S) in Gc\u2010domain. These findings highlight the significance of whole\u2010genome sequencing of indigenous strains for a better understanding of the CCHFV evolution in Pakistan.<\/jats:p>","DOI":"10.1002\/jmv.28604","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T10:05:27Z","timestamp":1677146727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Whole\u2010genome sequencing of Crimean\u2212Congo hemorrhagic fever virus circulating in Pakistan during 2022"],"prefix":"10.1002","volume":"95","author":[{"given":"Massab","family":"Umair","sequence":"first","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaira","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed A.","family":"Haider","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qasim","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"Hakim","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaheen","family":"Bibi","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aamer","family":"Ikram","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health  Chak Shehzad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2018-001114"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2003.12.035"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00752-06"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/trv050"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2010.01.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-016-3936-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.07.006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.11.208"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-201"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.29271\/jcpsp.2019.06.563"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30119-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008238"},{"key":"e_1_2_8_14_1","unstructured":"Field Epidemiology & Disease Surveillance Division National Institute of Health Islamabad. 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