{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T22:48:22Z","timestamp":1776725302856,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Skin rash is one of the most common complications during childhood. Viral agents play an essential role in the development of such symptoms. Present study aims to determine the prevalence and genetic variability of\u00a0Human Herpesvirus 6 and 7\u00a0(HHV\u20106 and\u00a0HHV\u20107) infections and their subtypes in children under 5 years of age with skin rash and negative for rubella and measles. We used serum and throat swap samples from 196 children with skin rash and fever. ELISA and IFA tests were performed to detect antibodies against HHV6\/7. Sequencing was performed using Sanger sequencing, and BioEdit and MEGA10 software were used for sequence analysis. According to the results, 66% and 40% of cases were positive for HHV\u20106 IgM and HHV\u20107 IgM, respectively. According to the molecular analysis, HHV\u20106 Nested\u2010PCR was positive in 18% of cases, however, HHV\u20107 Nested\u2010PCR was positive in 7.7% of cases. On the other hand, HHV\u20106 IgG and HHV\u20107 IgG were positive in 91% and 55% of study cases, respectively. For HHV\u20106, we found some genetic variabilities resulting in antigenic changes compared to reference strains. HHV\u20107 isolates showed no genetic differentiation and had a stable gene sequence. Based on the results, the detection of some cases of HHV6\/7 primary infection and the presence of specific symptoms of roseola in the study population needs continuous evaluation of HHV6\/7 frequency and distribution, also genetic variabilities of HHV6. This can pave the way for investigating HHV6 immune evasion and vaccine research and studying the relationship between viral genetic variations and other factors like disease severity. Furthermore, it is necessary to determine the relation between HHV6 genetic changes and latent infection to be considered in possible future vaccines and antiviral drug development.<\/jats:p>","DOI":"10.1002\/jmv.28608","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T10:05:45Z","timestamp":1677146745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Frequency evaluation and molecular characterization of HHV\u20106 and HHV\u20107 among children under 5 years with fever and skin rash"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3647-1619","authenticated-orcid":false,"given":"Mohsen","family":"Keshavarz","sequence":"first","affiliation":[{"name":"The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somayeh","family":"Ghasemi","sequence":"additional","affiliation":[{"name":"Department of Virology, School of Public Health Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaser","family":"Arjeini","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Production and Research Complex Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haideh","family":"Namdari","sequence":"additional","affiliation":[{"name":"Iranian Tissue Bank and Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Maleki","sequence":"additional","affiliation":[{"name":"Department of Virology, School of Public Health Tehran University of Medical Sciences Tehran 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