{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:48:49Z","timestamp":1777502929232,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004255","name":"Rajiv Gandhi University of Health Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004255","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007780","name":"Indian Institute of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exosomes are small extracellular vesicles secreted by cells and have a major role in cell\u2010to\u2010cell signaling. As dengue infection progresses from a mild to a severe form of infection, the exosome's microRNA (miRNA) composition might change, which may contribute to pathogenesis. In this study, a comprehensive analysis of serum exosomal miRNAs was performed and their involvement in dengue virus\u2010induced disease progression in an Indian cohort was assessed. Small RNA\u2010seq showed 50 differentially expressed exosomal miRNAs that were significantly dysregulated during dengue infection. After extensive validation, miR\u201096\u20105p was found to be significantly upregulated, whereas miR\u2010146a\u20105p was significantly downregulated with the progression of disease to severe form. Interestingly, a strong positive correlation was found between the expression levels of miR\u201096\u20105p and miR\u2010146a\u20105p and the platelet levels of the patients. Further, study of miR\u2010146a\u20105p showed that it regulates the expression of the proteins which are involved in the immune responses. These results suggest that miR\u201096\u20105p and miR\u2010146a\u20105p could be used as diagnostic and prognostic markers for dengue disease progression, in addition to the already available biochemical and pathological parameters.<\/jats:p>","DOI":"10.1002\/jmv.28614","type":"journal-article","created":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T07:29:16Z","timestamp":1677310156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Association of exosomal miR\u201096\u20105p and miR\u2010146a\u20105p with the disease severity in dengue virus infection"],"prefix":"10.1002","volume":"95","author":[{"given":"Aunji","family":"Pradhan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Aneja","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahana","family":"Ghosh","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Genomics  Kalyani India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Himadri","family":"Devvanshi","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepika","family":"C.","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risabh","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celil","family":"Ross","sequence":"additional","affiliation":[{"name":"St. John's Medical College  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pallavi","family":"Kshetrapal","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arindam","family":"Maitra","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Genomics  Kalyani India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0640-3586","authenticated-orcid":false,"given":"Saumitra","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"},{"name":"National Institute of Biomedical Genomics  Kalyani India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-803109-4.00015-5"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0357-z"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800098-4.00007-6"},{"key":"e_1_2_12_6_1","volume-title":". Research SPf, diseases TiT, diseases WHODoCoNT, epidemic WHO, Alert P. Dengue: Guidelines for diagnosis, treatment, prevention and control","author":"World Health Organization","year":"2009"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-020-02284-w"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00678.x"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2005.01.021"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82537-0"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v7062770"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.278"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11150"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-2069"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-0124"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.01.006"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485780"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20201267"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005981"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.01.001"},{"issue":"728","key":"e_1_2_12_23_1","article-title":"Regulation of host innate immunity by non\u2010coding RNAs during dengue virus infection","volume":"10","author":"Rajput R","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_12_24_1","doi-asserted-by":"crossref","unstructured":"MartinM. Cutadapt removes adapter sequences from high\u2010throughput sequencing reads.2011;17(1):3.","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr688"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1141"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-47"},{"issue":"1","key":"e_1_2_12_29_1","first-page":"2","article-title":"An overview of exosomes: from biology to emerging roles in immune response","volume":"47","author":"Avc\u0131 E","year":"2016","journal-title":"Acta Medica Cordoba"},{"issue":"5","key":"e_1_2_12_30_1","first-page":"2081","article-title":"Long non\u2010coding RNA CASC2 inhibits breast cancer cell growth and metastasis through the regulation of the miR\u201096\u20105p\/SYVN1 pathway","volume":"53","author":"Gao Z","year":"2018","journal-title":"Int J Oncol"},{"issue":"1","key":"e_1_2_12_31_1","first-page":"237","article-title":"Oncogenic miR\u201096\u20105p inhibits apoptosis by targeting the caspase\u20109 gene in hepatocellular carcinoma","volume":"53","author":"Iwai N","year":"2018","journal-title":"Int J Oncol"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184508"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2013.05.003"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-017-3516-9"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000854"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03618-4"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2018.09.006"},{"key":"e_1_2_12_38_1","first-page":"00915","article-title":"Cellular protein HuR regulates the switching of genomic RNA template for differential function during coxsackievirus B3 life cycle","author":"George B","year":"2021","journal-title":"J Virol"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1288"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.08.012"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2015.62"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5197592"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000411"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563215604001"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28614","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.28614","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T20:28:56Z","timestamp":1692649736000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/jmv.28614"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28614","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"2022-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28614"}}