{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:55:18Z","timestamp":1791352518314,"version":"4.3.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Yellow fever (YF) virus is a mosquito\u2010borne virus belonging to the <jats:italic>Flaviviridae<\/jats:italic> family that circulates in tropical and subtropical areas of Africa and South America. Despite the availability of an effective vaccine, YF remains a threat to travelers, residents of endemic areas, and unvaccinated populations. YF vaccination and natural infection both induce the production of neutralizing antibodies. Serological diagnostic methods detecting YF virus\u2010specific antibodies demonstrate high levels of cross\u2010reactivities with other flaviviruses. To date, the plaque reduction neutralization test (PRNT) is the most specific serological test for the differentiation of flavivirus infections and is considered the reference method for detecting YF neutralizing\u00a0antibodies and assessing the protective immune response following vaccination. In this study, we developed and validated a YF PRNT. We optimized different parameters including cell concentration and virus\u2010serum neutralization time period and then assessed the intra\u2010 and inter\u2010assay precisions, dilutability, specificity, and lower limit of quantification (LLOQ) using international standard YF serum, sera from vaccinees and human specimens collected through YF surveillance. The YF PRNT has shown good robustness and 100% of intra\u2010assay precision, 95.6% of inter\u2010assay precision, 100% of specificity, 100% of LLOQ, and 95.3% of dilutability. The test is, therefore, suitable for use in the YF diagnostic as well as evaluation of the YF vaccine neutralizing antibody response and risk assessment studies.<\/jats:p>","DOI":"10.1002\/jmv.28700","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T11:36:22Z","timestamp":1679571382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Performance assessment and validation of a plaque reduction neutralization test (PRNT) in support to yellow fever diagnostic and vaccine clinical trials"],"prefix":"10.1002","volume":"95","author":[{"given":"Moussa","family":"Dia","sequence":"first","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar  Dakar Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ndeye Sakha","family":"Bob","sequence":"additional","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar  Dakar 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