{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:42:06Z","timestamp":1790185326181,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["JCYJ20210324112202006"],"award-info":[{"award-number":["JCYJ20210324112202006"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171378"],"award-info":[{"award-number":["82171378"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Herpetic\u2010related neuralgia (HN) caused by varicella\u2010zoster virus (VZV) infection is one of the most typical and common neuropathic pain in the clinic. However, the potential mechanisms and therapeutic approaches for the prevention and treatment of HN are still unclear. This study aims to provide a comprehensive understanding of the molecular mechanisms and potential therapeutic targets of HN. We used an HSV\u20101 infection\u2010induced HN mouse model and screened the differentially expressed genes (DEGs) in the DRG and spinal cord using an RNAseq technique. Moreover, bioinformatics methods were used to figure out the signaling pathways and expression regulation patterns of the DEGs enriched. In addition, quantitative real\u2010time RT\u2010PCR and western blot were carried out to further confirm the expression of DEGs. HSV\u20101 inoculation in mice resulted in mechanical allodynia, thermal hyperalgesia, and cold allodynia, following the infection of HSV\u20101 in both DRG and spinal cord. Besides, HSV\u20101 inoculation induced an up\u2010regulation of ATF3, CGRP, and GAL in DRG and activation of astrocytes and microglia in the spinal cord. Moreover, 639 genes were upregulated, 249 genes were downregulated in DRG, whereas 534 genes were upregulated and 12 genes were downregulated in the spinal cord of mice 7 days after HSV\u20101 inoculation. GO and KEGG enrichment analysis suggested that immune responses and cytokine\u2010cytokine receptor interaction are involved in DRG and spinal cord neurons in mice after HSV\u20101 infection. In addition, CCL5 and its receptor CCR5 were significantly upregulated in DRG and spinal cord upon HSV\u20101 infection in mice. And blockade of CCR5 exhibited a significant analgesic effect and suppressed the upregulation of inflammatory cytokines in DRG and spinal cord induced by HSV\u20101 infection in mice. HSV\u20101 infection\u2010induced allodynia and hyperalgesia in mice through dysregulation of immune response and cytokine\u2010cytokine receptor interaction mechanism. Blockade of CCR5 alleviated allodynia and hyperalgesia probably through the suppression of inflammatory cytokines. Therefore, CCR5 could be a therapeutic target for the alleviation of HSV\u20101 infection\u2010induced HN.<\/jats:p>","DOI":"10.1002\/jmv.28718","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T12:09:42Z","timestamp":1681474182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["HSV\u20101 infection\u2010induced herpetic neuralgia involves a CCL5\/CCR5\u2010mediated inflammation mechanism"],"prefix":"10.1002","volume":"95","author":[{"given":"Songbin","family":"Wu","sequence":"first","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaomin","family":"Yang","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongzhen","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyuan","family":"Ba","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donglin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2543-4369","authenticated-orcid":false,"given":"Wuping","family":"Sun","sequence":"additional","affiliation":[{"name":"Shenzhen Municipal Key Laboratory for Pain Medicine, Department of Pain Medicine, National Key Clinic of Pain Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital The 6th Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"issue":"10","key":"e_1_2_12_2_1","first-page":"656","article-title":"Herpes zoster and postherpetic neuralgia: prevention and management","volume":"96","author":"Saguil A","year":"2017","journal-title":"Am Fam Physician"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpainsymman.2004.01.014"},{"key":"e_1_2_12_4_1","first-page":"189","article-title":"A guide to preclinical models of zoster\u2010associated pain and postherpetic neuralgia","volume":"438","author":"Warner BE","year":"2023","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200107000-00034"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000492"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2014-004833"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000307"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40122-019-0127-z"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000500482"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40122-021-00303-7"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2020.112942"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08998280.2020.1767461"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-019-4446-0"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.02.024"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3215"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix508"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-9-2433"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.03.046"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1173"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1064"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens2020364"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009689"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040634"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03170"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00240-2"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.08154FP"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5198(19)30567-0"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1384"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061029798"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(98)00050-9"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709250104"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3588-08.2008"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-022-10047-8"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2016.05.003"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2017.01.014"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.6.2780-2788.2002"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81339-0"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2021.741054"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.09.035"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13760-018-1006-9"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn025"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-323"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2021.665931"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl167"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2016.04.024"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.615327"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.02966.x"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.10.024"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.26.6.596"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-019-08581-3"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/17448069221106167"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000000190"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01245"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.8.3542"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0229570"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.1.264-271.1996"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T07:57:43Z","timestamp":1692259063000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1002\/jmv.28718"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28718","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"2022-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28718"}}