{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:41:45Z","timestamp":1787578905919,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The best method for monitoring cytomegalovirus (CMV)\u2010specific cell\u2010mediated immunity (CMV\u2010CMI) among high\u2010risk kidney transplant (KT) recipients remains uncertain. We assessed CMV\u2010CMI by intracellular cytokine staining (ICS) by flow cytometry and a commercial interferon (IFN)\u2010\u03b3 release assay (QuantiFERON\u00ae\u2010CMV [QTF\u2010CMV]) at posttransplant months 3, 4, and 5 in 53 CMV\u2010seropositive KT recipients that had received induction therapy with antithymocyte globulin (ATG) and a 3\u2010month course of valganciclovir prophylaxis. The discriminative capacity (areas under receiver operating characteristics curve [auROCs]) and diagnostic accuracy to predict immune protection against CMV infection from the discontinuation of prophylaxis to month 12 were compared between both methods. There was significant although moderate correlations between CMV\u2010specific IFN\u2010\u03b3\u2010producing CD8<jats:sup>+<\/jats:sup> T\u2010cell counts enumerated by ICS and IFN\u2010\u03b3 levels by QTF\u2010CMV at months 3 (rho: 0.493; <jats:italic>p<\/jats:italic>\u2009=\u20090.005) and 4 (rho: 0.440; <jats:italic>p<\/jats:italic>\u2009=\u20090.077). The auROCs for CMV\u2010specific CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cells by ICS were nonsignificantly higher than that of QTF\u2010CMV (0.696 and 0.733 vs. 0.678; <jats:italic>p<\/jats:italic>\u2009=\u20090.900 and 0.692, respectively). The optimal cut\u2010off of \u22650.395 CMV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cells yielded a sensitivity of 86.4%, specificity of 54.6%, positive predictive value of 79.2% and negative predictive value of 66.7% to predict protection. The corresponding estimates for QTF\u2010CMV (IFN\u2010\u03b3 levels \u22650.2\u2009IU\/mL) were 78.9%, 37.5%, 75.0%, and 42.9%, respectively. The enumeration of CMV\u2010specific IFN\u2010\u03b3\u2010producing CD8<jats:sup>+<\/jats:sup> T\u2010cells at the time of cessation of prophylaxis performed slightly better than the QTF\u2010CMV assay to predict immune protection in seropositive KT recipients previously treated with ATG.<\/jats:p>","DOI":"10.1002\/jmv.28733","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T11:14:47Z","timestamp":1681384487000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comparison of intracellular cytokine staining versus an ELISA\u2010based assay to assess CMV\u2010specific cell\u2010mediated immunity in high\u2010risk kidney transplant recipients"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0315-8001","authenticated-orcid":false,"given":"Mario","family":"Fern\u00e1ndez\u2010Ruiz","sequence":"first","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine, School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9356-8102","authenticated-orcid":false,"given":"Natalia","family":"Redondo","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5852-5884","authenticated-orcid":false,"given":"Patricia","family":"Parra","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8261-6057","authenticated-orcid":false,"given":"Tamara","family":"Ruiz\u2010Merlo","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2150-5748","authenticated-orcid":false,"given":"Isabel","family":"Rodr\u00edguez\u2010Goncer","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Polanco","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5333-7529","authenticated-orcid":false,"given":"Francisco","family":"L\u00f3pez\u2010Medrano","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine, School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3446-1991","authenticated-orcid":false,"given":"Rafael","family":"San Juan","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine, School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-4110","authenticated-orcid":false,"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Microbiology, Hospital Cl\u00ednico Universitario Instituto de Investigaci\u00f3n Sanitaria INCLIVA Valencia Spain"},{"name":"Department of Microbiology, School of Medicine University of Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-1364","authenticated-orcid":false,"given":"Amado","family":"Andr\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine Universidad Complutense Madrid Spain"},{"name":"Department of Nephrology, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9520-8255","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Aguado","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases, Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12)  Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine, School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis818"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trre.2016.04.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/cti.2014.3"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/315347"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2006.00199.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15793"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05633-11"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"195","article-title":"Comparison of the T\u2010cell response to human cytomegalovirus (HCMV) as detected by cytokine flow cytometry and QuantiFERON\u2010CMV assay in HCMV\u2010seropositive kidney transplant recipients","volume":"41","author":"Gabanti E","year":"2018","journal-title":"New Microbiol"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002191"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2010.330"},{"key":"e_1_2_10_12_1","unstructured":"QuantiFERON\u00ae\u2010CMV assay package insert (1075110 Rev. 05). QIAGEN GmbH Hilden Germany (February 2018)."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw668"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3"},{"issue":"4","key":"e_1_2_10_15_1","first-page":"247","article-title":"Predictive value of human cytomegalovirus (HCMV) T\u2010cell response in the control of HCMV infection by seropositive solid\u2010organ transplant recipients according to different assays and stimuli","volume":"39","author":"Gabanti E","year":"2016","journal-title":"New Microbiol"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189488"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01716-21"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.05.010"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000746"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13977"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab574"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2017.17.5.317"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200526023"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-022-04422-7"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02618.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2006.01488.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/tri.12321"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199711270-00010"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31823c1cd4"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01202-2"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28733","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T07:58:08Z","timestamp":1692259088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1002\/jmv.28733"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28733","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"2023-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28733"}}