{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T06:09:54Z","timestamp":1786169394572,"version":"build-2736575974"},"reference-count":79,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:00:00Z","timestamp":1683072000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The vaginal microbiome has emerged as potentially influencing the natural history of Human Papillomavirus (HPV) infections and their clinical impact. We aimed to characterize the vaginal microbiome in samples from 807 high\u2010risk HPVs (Hr\u2010HPV) positive women with a mean age of 41.45\u2009\u00b1\u200910.79 years who participated in the Regional Cervical Cancer Screening Program from the Northern Region of Portugal. Microbiome analysis was performed with commercial kits for the detection of 21 microorganisms. The most frequent microorganisms were <jats:italic>Ureaplasma parvum<\/jats:italic> (52.5%), <jats:italic>Gardnerella vaginalis<\/jats:italic> (GV) (34.5%), <jats:italic>Atopobium vaginae<\/jats:italic> (AV) (32.6%), Lacto (30.7%), and <jats:italic>Mycoplasma hominis<\/jats:italic> (MH) (23.5%). The distribution according to age reveals that MH, Mega1, GV, BVab2, AV, and Mob were more prevalent in women older than 41 years of age (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.050), while Lacto is significantly decreased in this group (23.5% vs. 39.4%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001; RR\u2009=\u20090.47). The risk analysis showed that Hr\u2010HPV\u201016\/\u201018 and Hr\u2010HPV\u20109val genotypes are associated with an increased risk of developing cervical abnormalities, while Lacto (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001; odd ratio [OR]\u2009=\u20090.33), GV (<jats:italic>p<\/jats:italic>\u2009=\u20090.0111; OR\u2009=\u20090.41), AV (<jats:italic>p<\/jats:italic>\u2009=\u20090.033; OR\u2009=\u20090.53) and Mob (<jats:italic>p<\/jats:italic>\u2009=\u20090.022; OR\u2009=\u20090.29) are associated with protection. Similar results were found for the risk of development atypical squamous cells cannot exclude HSIL\/high\u2010grade squamous intraepithelial lesion. Overall, the multivariate analysis confirmed that lactobacillus and bacteria associated with bacterial vaginosis (GV, AV, and Mob) are associated with protection against the development of cervical abnormalities. This study provides important data to be included in the future management of risk stratification for Hr\u2010HPV\u2010positive women.<\/jats:p>","DOI":"10.1002\/jmv.28762","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T13:48:59Z","timestamp":1683121739000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Impact of cervicovaginal microbiome on the risk of cervical abnormalities development"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4348-0596","authenticated-orcid":false,"given":"Andreia","family":"Ros\u00e1rio","sequence":"first","affiliation":[{"name":"Molecular Oncology &amp; Viral Pathology Group, Research Center (CI\u2010IPOP)\/RISE@CI\u2010IPOP (Health Research Network) Portuguese Oncology Institute of Porto (IPO Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC) Porto Portugal"},{"name":"Clinical Pathology 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