{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:57:09Z","timestamp":1753869429709,"version":"3.41.2"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:00:00Z","timestamp":1683072000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>People are expected to have been previously vaccinated with a Vaccinia\u2010based vaccine, as until 1980 smallpox vaccination was a standard protocol in China. It is unclear whether people with smallpox vaccine still have antibody against vaccinia virus (VACV) and cross\u2010antibody against monkeypox virus (MPXV). Herein, we assessed the binding antibodies with antigen of VACV\u2010A33 and MPXV\u2010A35 in the general population and HIV\u20101 infected patients. Firstly, we detected VACV antibody with A33 protein to evaluate the efficiency of smallpox vaccination. The result show that 29% (23 of 79) of hospital staff (age \u2265 42 years) and 63% (60 of 95) of HIV\u2010positive patients (age \u2265 42 years) from Guangzhou Eighth People's Hospital were able to bind A33. However, among the subjects below 42 years of age, 1.5% (3\/198) of the hospital volunteer samples and 1% (1\/104) of the samples from HIV patients were positive for antibodies against A33 antigen. Then, we assessed the specific cross\u2010reactive antibodies against MPXV A35 protein. 24% (19 of 79) hospital staff (age\u232a = 42 years) and 44% (42 of 95) of HIV\u2010positive patients (age\u232a = 42 years) were positive. 98% (194\/198) of the hospital staff and 99% (103\/104) of the HIV patients had no A35\u2010binding antibodies. Further, we found significant sex differences for the reactivity to A35 antigen were observed in HIV population, but no significant sex differences in hospital staff. Further, we analyzed the positivity rate of anti\u2010A35 antibody of men who have sex with men (MSM) and non\u2010MSM in HIV patients (age\u232a = 42years). We found that 47% of no\u2010MSM population and 40% of MSM population were positive for A35 antigen, with no significant difference. Lastly, we found only 59 samples were positive for anti\u2010A33 IgG and anti\u2010A35 IgG in all participants. Together, we demonstrated A33 and A35 antigens binding antibodies were detected in HIV patients and general population who were older than 42 years, and cohort studies only provided data of serological detection to support early response to monkeypox outbreak.<\/jats:p>","DOI":"10.1002\/jmv.28763","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T07:50:26Z","timestamp":1683100226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Serological responses to smallpox A33 antigen and monkeypox A35 antigen in healthy people and people living with HIV\u20101 from Guangzhou, China"],"prefix":"10.1002","volume":"95","author":[{"given":"Jun","family":"Dai","sequence":"first","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingyin","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Tang","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linghua","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1757-1411","authenticated-orcid":false,"given":"Haisheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imj.2022.07.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.ade8470"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virs.2022.07.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00775-4"},{"key":"e_1_2_7_6_1","article-title":"A35R and H3L are serological and B cell markers for monkeypox infection","author":"Yefet R","year":"2022","journal-title":"medRxiv"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virs.2022.10.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/17.3.643"},{"key":"e_1_2_7_9_1","article-title":"Analogous humoral antigen recognition between monkeypox\u2010infected and smallpox\u2010vaccinated individuals","author":"Otter AD","year":"2022","journal-title":"medRxiv"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm917"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-02090-w"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2207323"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T11:03:14Z","timestamp":1705921394000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/jmv.28763"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28763","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2023-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28763"}}