{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:21:13Z","timestamp":1791483673041,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012285","name":"Departement Economie, Wetenschap en Innovatie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011878","name":"Vlaamse regering","doi-asserted-by":"publisher","award":["G096222N"],"award-info":[{"award-number":["G096222N"]}],"id":[{"id":"10.13039\/501100011878","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>The risk of infection after exposure to clade IIb mpox virus (MPXV) is unknown, and potential presymptomatic shedding of MPXV remains to be demonstrated.\u00a0High\u2010risk contacts of mpox patients were followed\u2010up in a prospective longitudinal cohort study. Individuals reporting sexual contact, &gt;15\u2009min skin\u2010to\u2010skin contact, or living in the same household with an mpox patient were recruited in a sexual health clinic in Antwerp, Belgium. Participants kept a symptom diary, performed daily self\u2010sampling (anorectal, genital, and saliva), and presented for weekly clinic visits for physical examination and sampling (blood and oropharyngeal). Samples were tested for MPXV by PCR.\u00a0Between June 24 and July 31, 2022, 25 contacts were included, of which 12\/18 (66.0%) sexual and 1\/7 (14.0%) nonsexual contacts showed evidence of infection by MPXV\u2010PCR. Six cases had typical mpox symptoms. Viral DNA was detected as early as 4\u00a0days before symptom onset in 5\u00a0of them. In 3\u00a0of these cases, replication\u2010competent virus was demonstrated in the presymptomatic phase.\u00a0These findings confirm the existence of presymptomatic shedding of replication\u2010competent MPXV and emphasize the high risk of transmission during sexual contact. Sexual contacts of mpox cases should abstain from sex during the incubation period, irrespective of symptoms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.28769","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T10:12:53Z","timestamp":1683195173000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Presymptomatic viral shedding in high\u2010risk mpox contacts: A prospective cohort study"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2227-8874","authenticated-orcid":false,"given":"Isabel","family":"Brosius","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Van Dijck","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"},{"name":"Laboratory of Medical Microbiology University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Coppens","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen","family":"Vandenhove","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2314-2258","authenticated-orcid":false,"given":"Eugene","family":"Bangwen","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fien","family":"Vanroye","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Verschueren","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"The ITM MPOX Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Zange","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Bugert","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Michiels","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Bottieau","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Soentjens","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"van Griensven","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Kenyon","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"},{"name":"Division of Infectious Diseases and HIV Medicine University of Cape Town Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin K.","family":"Ari\u00ebn","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Institute of Tropical Medicine Antwerp Belgium"},{"name":"Department of Biomedical Sciences University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Van Esbroeck","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"Vercauteren","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5616-8548","authenticated-orcid":false,"given":"Laurens","family":"Liesenborghs","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,4]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"Geneva: World Health Organization. 2022 Mpox (Monkeypox) outbreak: global trends [Internet].https:\/\/worldhealthorg.shinyapps.io\/mpx_global\/"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2208860"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00586-2"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00639-9"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2207323"},{"key":"e_1_2_12_7_1","unstructured":"World Health Organization. Clinical management and infection prevention and control for monkeypox. Interim rapid response guidance.2022;(June):76."},{"key":"e_1_2_12_8_1","unstructured":"European Centre for Disease Prevention and Control (ECDC). Considerations for contact tracing during the monkeypox outbreak in Europe 2022."},{"key":"e_1_2_12_9_1","article-title":"Retrospective detection of asymptomatic monkeypox virus infections among male sexual health clinic attendees in Belgium","volume":"16","author":"De Baetselier I","year":"2022","journal-title":"Nat Med"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac723"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/M22-2183"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00379-2"},{"key":"e_1_2_12_13_1","doi-asserted-by":"crossref","DOI":"10.1136\/bmj-2022-073153","article-title":"Transmission dynamics of monkeypox in the United Kingdom: contact tracing study","volume":"379","author":"Ward T","year":"2022","journal-title":"BMJ"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.02.060"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.o2504"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2018-001349"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac889"},{"key":"e_1_2_12_18_1","unstructured":"World Health Organization. Self\u2010collection of samples for sexually transmitted infections (\u200e\u200eSTIs)\u200e\u200e [Internet].https:\/\/www.who.int\/publications\/i\/item\/WHO-SRH-20.10"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-02004-w"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7136e1"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T06:03:28Z","timestamp":1705903408000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/jmv.28769"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28769","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2023-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28769"}}