{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:00:14Z","timestamp":1784674814186,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Whole\u2010genome sequencing (WGS) has been widely used for the genomic characterization and the phylogenesis of mpox virus (MPXV) 2022 multi\u2010country outbreak. To date, no evidence has been reported on intra\u2010host evolution within samples collected over time from a single patient with long\u2010term infection. Fifty\u2010one samples were collected from five patients at different time points post\u2010symptom onset. All samples were confirmed as MPXV DNA positive, amplified by a multiplexed PCR amplicon, and sequenced by WGS. Complete MPXV genomes were assembled by reference mapping and then aligned to perform phylogenetic and hierarchical clustering analysis. Large intra\u2010host variability was observed among the MPXV genomes sequenced from samples of two immunocompromised with advanced HIV\u20101 infection patients with prolonged MPXV shedding. Overall, 20 nucleotide mutations were identified in the 32 genomes from HIV patients, differently distributed in samples collected from different tissues and at different time points. No sequence compartmentalization nor variation was observed in the three patients with rapid viral clearance. MPXV exhibits adaptation to changing environments within the infected host and consequently demonstrates tissue compartmentalization. Further studies are needed to elucidate the role of this adaptation in forming a pool of genetic variability and contributing to viral persistence and its clinical implications.<\/jats:p>","DOI":"10.1002\/jmv.28791","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T06:09:24Z","timestamp":1684994964000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Temporal intra\u2010host variability of mpox virus genomes in multiple body tissues"],"prefix":"10.1002","volume":"95","author":[{"given":"Martina","family":"Rueca","sequence":"first","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases \u201cL. 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