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However, elucidating the molecular basis for pulmonary immune response alterations is challenging owing to the virus's heterogeneous distribution within complex tissue structure. Here, we revealed the spatial transcriptomic profiles of pulmonary microstructures at the SARS\u2010CoV\u20102 infection site in the nine cynomolgus macaques upon inoculation with the Delta and Omicron variants. Delta\u2010 and Omicron\u2010infected lungs had upregulation of genes involved in inflammation, cytokine response, complement, cell damage, proliferation, and differentiation pathways. Depending on the tissue microstructures (alveoli, bronchioles, and blood vessels), there were differences in the types of significantly upregulated genes in each pathway. Notably, a limited number of genes involved in cytokine and cell damage response were differentially expressed between bronchioles of the Delta\u2010 and Omicron\u2010infection groups. These results indicated that despite a significant antigenic shift in SARS\u2010CoV\u20102, the host immune response mechanisms induced by the variants were relatively consistent, with limited transcriptional alterations observed only in large airways. This study may aid in understanding the pathogenesis of SARS\u2010CoV\u20102 and developing a clinical strategy for addressing immune dysregulation by identifying potential transcriptional biomarkers within pulmonary microstructures during infection with emerging variants.<\/jats:p>","DOI":"10.1002\/jmv.28847","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T19:37:48Z","timestamp":1686166668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparative spatial transcriptomic profiling of severe acute respiratory syndrome coronavirus 2 Delta and Omicron variants infections in the lungs of cynomolgus macaques"],"prefix":"10.1002","volume":"95","author":[{"given":"Taehwan","family":"Oh","sequence":"first","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB) National Primate Research Centre Cheongju Chungcheongbuk Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Green","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB) National Primate Research Centre Cheongju Chungcheongbuk Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Ho","family":"Baek","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB) National Primate Research Centre Cheongju Chungcheongbuk Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YoungMin","family":"Woo","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB) National Primate Research Centre Cheongju Chungcheongbuk Republic of Korea"},{"name":"KRIBB School of Bioscience Korea University of Science &amp; 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