{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:35:36Z","timestamp":1792488936072,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Information on the immune response during the mpox virus (MPXV) infection is still scarce or limited to past studies when cross\u2010reactive immunity from smallpox vaccination was predominant. Here, we describe the short\u2010term kinetics of the antibody response in patients with acute MPXV infection during the 2022 multicountry outbreak.\u00a0A total of 64 samples from 18 MPXV\u2010positive patients were longitudinally collected from the day of symptom onset (DSO) up to 20 days after and tested for anti\u2010MPXV\u00a0immunoglobulin G (IgG), IgM, IgA, and neutralizing antibodies (nAb) using the whole\u2010live virus isolated in May 2022. IgG, IgM, and IgA were detected as early as 4 DSO (median time of seroconversion 7.5 DSO for IgG, 8 DSO for IgM and IgA). Anti\u2010MPXV nAb were detectable in samples collected as early as 1 week after symptoms, with stable levels up to 20 DSO. After 2 weeks, IgG and nAb reached high titers. No significant differences were observed regardless of status of smallpox vaccination, human immunodeficiency virus positivity, or disease severity. Significant lower levels of IgM and IgG were observed in the patients treated with antivirals.\u00a0These results contribute to extending the knowledge of the MPXV infection and the antibody response in a population with no historic smallpox vaccination.<\/jats:p>","DOI":"10.1002\/jmv.28851","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T05:50:38Z","timestamp":1686203438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Profiling the acute phase antibody response against mpox virus in patients infected during the 2022 outbreak"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0640-8667","authenticated-orcid":false,"given":"Francesca","family":"Colavita","sequence":"first","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-4243","authenticated-orcid":false,"given":"Giulia","family":"Matusali","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0240-7504","authenticated-orcid":false,"given":"Valentina","family":"Mazzotta","sequence":"additional","affiliation":[{"name":"Clinical and Research Infectious Diseases Department National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Bettini","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Lapa","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Meschi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Francalancia","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Pinnetti","sequence":"additional","affiliation":[{"name":"Clinical and Research Infectious Diseases Department National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3962-3646","authenticated-orcid":false,"given":"Licia","family":"Bordi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klizia","family":"Mizzoni","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Coen","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Girardi","sequence":"additional","affiliation":[{"name":"Scientific Direction National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Vaia","sequence":"additional","affiliation":[{"name":"General Direction National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Nicastri","sequence":"additional","affiliation":[{"name":"Clinical and Research Infectious Diseases Department National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Antinori","sequence":"additional","affiliation":[{"name":"Clinical and Research Infectious Diseases Department National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Maggi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9120380"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032299"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.38.1800509"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00148-07"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015547"},{"key":"e_1_2_10_7_1","article-title":"Clinical characterization of human monkeypox infections in the Democratic Republic of the Congo","author":"Pittman PR","year":"2022","journal-title":"MedRxiv"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.7.867-872.2005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00379-2"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106102"},{"key":"e_1_2_10_11_1","unstructured":"ECDC. Factsheet for health professionals on mpox (monkeypox).2022.\u00a0Accessed date October 25 2022."},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.28.2200503"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2810.221107"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.09.049"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-02090-w"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00662-4"},{"key":"e_1_2_10_17_1","article-title":"Mpox vaccine and infection\u2010driven human immune signatures","author":"Cohn H","year":"2023","journal-title":"medRxiv"},{"key":"e_1_2_10_18_1","unstructured":"World Health Organization. Laboratory testing for the monkeypox virus: interim guidance.2022.https:\/\/www.who.int\/publications\/i\/item\/WHO-MPX-laboratory-2022.1"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12459"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.01009.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0474-z"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00228-6"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac377"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2022.11.001"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01436-2"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111260"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104424"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28851","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T15:46:49Z","timestamp":1692287209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/jmv.28851"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28851","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"2023-02-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28851"}}