{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T19:44:23Z","timestamp":1779392663536,"version":"3.53.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monkeypox (MPOX) is a zoonotic disease that affects humans and other primates, resulting in a smallpox\u2010like illness. It is caused by monkeypox virus (MPXV), which belongs to the <jats:italic>Poxviridae<\/jats:italic> family. Clinically manifested by a range of cutaneous and systemic findings, as well as variable disease severity phenotypes based on the genetic makeup of the virus, the cutaneous niche and respiratory mucosa are the epicenters of MPXV pathogenicity. Herein, we describe the ultrastructural features of MPXV infection in both human cultured cells and cutaneous clinical specimens collected during the 2022\u20132023 MPOX outbreak in New York City that were revealed through electron microscopy. We observed typical enveloped virions with brick\u2010shaped morphologies that contained surface protrusions, consistent with the classic ultrastructural features of MPXV. In addition, we describe morpho\u2010functional evidence that point to roles of distinct cellular organelles in viral assembly during clinical MPXV infection. Interestingly, in skin lesions, we found abundant melanosomes near viral assembly sites, particularly in the vicinity of mature virions, which provides further insight into virus\u2010host interactions at the subcellular level that contribute to MPXV pathogenesis. These findings not only highlight the importance of electron microscopic studies for further investigation of this emerging pathogen but also in characterizing MPXV pathogenesis during human infection.<\/jats:p>","DOI":"10.1002\/jmv.28878","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T04:28:55Z","timestamp":1686889735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Genomic and ultrastructural analysis of monkeypox virus in skin lesions and in human\/animal infected cells reveals further morphofunctional insights into viral pathogenicity"],"prefix":"10.1002","volume":"95","author":[{"given":"Alberto","family":"Paniz\u2010Mondolfi","sequence":"first","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai New York 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College of Public Health and Health Professions, Emerging Pathogens Institute University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph Patrick","family":"McGrail","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, Public Health and Microbiology Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana","family":"Kasminskaya","sequence":"additional","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz H.","family":"Patino","sequence":"additional","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai New York 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