{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:31:36Z","timestamp":1791869496133,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serum samples were collected from 54 hepatitis B e antigen (HBeAg)\u2010positive\u00a0Chinese patients infected with hepatitis B virus (HBV)\u00a0subgenotype B2 or C2. They were compared for transmission efficiency using same volume of samples or infectivity using same genome copy number. Adding polyethylene glycol (PEG)\u00a0during inoculation did not increase infectivity of fresh samples but markedly increased infectivity following prolonged sample storage. Differentiated HepaRG cells infected without PEG produced more hepatitis B surface antigen (HBsAg)\u00a0and higher HBsAg\/HBeAg\u00a0ratio than sodium taurocholate cotransporting polypeptide (NTCP)\u2010reconstituted\u00a0HepG2 cells infected with PEG. They better supported replication of core promoter mutant in contrast to wild\u2010type (WT)\u00a0virus by HepG2\/NTCP cells. Overall, subgenotype C2 samples had higher viral load than B2 samples, and in general produced more HBeAg, HBsAg, and replicative DNA following same\u2010volume inoculation. Precore mutant was more prevalent in subgenotype B2 and had reduced transmission efficiency. When same genome copy number of viral particles was inoculated, viral signals were not necessarily higher for three WT C2 isolates than four WT B2 isolates. Using viral particles generated from cloned HBV genome, three WT C2 isolates showed slightly reduced infectivity than three B2 isolates. In conclusion, subgenotype C2 serum samples had higher transmission efficiency than B2 isolates in association with higher viral load and lower prevalence of precore mutant, but not necessarily higher infectivity. PEG\u2010independent infection by HBV viremic serum samples is probably attributed to a labile host factor.<\/jats:p>","DOI":"10.1002\/jmv.28879","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T11:22:15Z","timestamp":1686741735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Clinical isolates of hepatitis B virus genotype C have higher in vitro transmission efficiency than genotype B isolates"],"prefix":"10.1002","volume":"95","author":[{"given":"Jing","family":"Li","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Department of Infectious Diseases, National Medical Center for Infectious Diseases, Huashan Hospital Fudan University Shanghai China"},{"name":"Liver Research Center, Division of Gastroenterology Rhode Island Hospital and Warren Alpert Medical School of Brown University Providence Rhode 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