{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:37:00Z","timestamp":1775018220321,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T00:00:00Z","timestamp":1688256000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001849","name":"Defence Research and Development Organisation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001849","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mpox (previously known as Monkeypox) has recently re\u2010emerged, primarily through human\u2010to\u2010human transmission in non\u2010endemic countries including India. Virus isolation is still considered as the gold standard for diagnosis of viral infections. Here, the qPCR positive skin lesion sample from a patient was inoculated in Vero E6 cell monolayer. Characteristic cytopathic effect\u00a0exhibiting typical cell rounding and detachment was observed at passage\u201002. The virus isolation was confirmed by qPCR. The replication kinetics of the isolate was determined that revealed maximum viral titre of log 6.3 PFU\/mL at 72\u2009h postinfection. Further, whole genome analysis through next generation sequencing revealed that the Mpox virus (MPXV) isolate is characterized by several unique SNPs and INDELs. Phylogenetically, it belonged to A.2 lineage of clade IIb, forming a close group with all other Indian MPXV along with few from USA, UK, Portugal, Thailand and Nigeria. This study reports the first successful isolation and phenotypic and genotypic characterization of MPXV from India.<\/jats:p>","DOI":"10.1002\/jmv.28911","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T05:25:25Z","timestamp":1688361925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Isolation and characterization of emerging Mpox virus from India"],"prefix":"10.1002","volume":"95","author":[{"given":"Sushil Kumar","family":"Sharma","sequence":"first","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paban Kumar","family":"Dash","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram Govind","family":"Yadav","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambuj","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Menon","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyoti S.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shashi","family":"Sharma","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suman","family":"Dhankher","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Dhiman","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Divya","family":"Kumari","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha","family":"Shukla","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vineet","family":"Relhan","sequence":"additional","affiliation":[{"name":"Maulana Azad Medical College and Lok Nayak Hospital  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suresh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Maulana Azad Medical College and Lok Nayak Hospital  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manmohan","family":"Parida","sequence":"additional","affiliation":[{"name":"Division of Virology Defence Research &amp; Development Establishment (DRDE)  Gwalior India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,2]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Multi\u2010country outbreak of monkeypox External situation report #8. October 19 2022:https:\/\/www.who.int\/publications\/m\/item\/multi-country-outbreak-of-monkeypox--external-situation-report--8---19-october-2022"},{"key":"e_1_2_11_3_1","unstructured":"World Health Organization. Disease Outbreak News; Monkeypox\u2013United Kingdom of Great Britain and Northern Ireland. Accessed May 16 2022.\u00a0https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2022-DON381"},{"key":"e_1_2_11_4_1","unstructured":"Monkeypox Who.int. Mpox (monkeypox): WHO Fact sheet. Accessed\u00a0October 26 2022.https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/monkeypox"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2019.03.001"},{"key":"e_1_2_11_6_1","volume-title":"StatPearls","author":"Moore MJ","year":"2022"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.05.030"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1959.tb00328.x"},{"key":"e_1_2_11_9_1","first-page":"593","article-title":"A human infection caused by monkeypox virus in Basankusu territory, democratic republic of the Congo","volume":"46","author":"Ladnyj ID","year":"1972","journal-title":"Bull World Health Organ"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90748-8"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00148-07"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/498155"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.2.1.17"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed1010008"},{"key":"e_1_2_11_15_1","first-page":"465","article-title":"Human monkeypox: secondary attack rates","volume":"66","author":"Jezek Z","year":"1988","journal-title":"Bull World Health Organ"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.428"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0168"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028295"},{"key":"e_1_2_11_19_1","first-page":"165","article-title":"Human monkeypox, 1970\u201079","volume":"58","author":"Breman JG","year":"1980","journal-title":"Bull World Health Organ"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit703"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12111257"},{"key":"e_1_2_11_22_1","unstructured":"Guidelines for management of monkeypox disease Ministry of Health and Family Welfare Government of India.2022.\u00a0https:\/\/main.mohfw.gov.in\/sites\/default\/files\/Guidelines%20for%20Management%20of%20Monkeypox%20Disease.pdf"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.105"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2204717119"},{"key":"e_1_2_11_25_1","unstructured":"WelkersM JongesM van denOudenA. Monkeypox virus whole genome sequencing using combination of NextGenPCR and Oxford Nanopore. 2022.\u00a0https:\/\/protocols.io\/view\/monkeypox-virus-whole-genome-sequencing-using-comb-ccc7sszn"},{"key":"e_1_2_11_26_1","unstructured":"Porechop v0.2.3. Adapter trimmer for Oxford Nanopore reads.2017.\u00a0https:\/\/github.com\/rrwick\/Porechop."},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_11_32_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1029\/96PA03934"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010141"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.14857"},{"key":"e_1_2_11_37_1","unstructured":"Vaccines and immunization for monkeypox: Interim guidance 24 August 2022.https:\/\/www.who.int\/publications\/i\/item\/WHO-MPX-Immunization-2022.2-eng"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2022.102386"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01907-y"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.add4153"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.28911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T09:24:04Z","timestamp":1696411444000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.28911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/jmv.28911"],"URL":"https:\/\/doi.org\/10.1002\/jmv.28911","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"2023-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e28911"}}