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There are no approved ZIKV\u2010specific vaccines or drugs for the clinical treatment of the infection. Currently, there are no approved ZIKV\u2010specific vaccines or drugs for the clinical treatment of the infection. In this study, we investigated the antiviral potential of aloperine, a quinolizidine alkaloid, against ZIKV infection in vivo and in vitro. Our results demonstrate that aloperine effectively inhibits ZIKV infection in vitro, with a low nanomolar half maximal effective concentration (EC<jats:sub>50<\/jats:sub>). Specifically, aloperine strongly protected cells from ZIKV multiplication, as indicated by decreased expression of viral proteins and virus titer. Our further investigations using the time\u2010of\u2010drug\u2010addition assay, binding, entry, and replication assays, detection of ZIKV strand\u2010specific RNA, the cellular thermal shift assay, and molecular docking revealed that aloperine significantly inhibits the replication stage of the ZIKV life cycle by targeting the domain RNA\u2010dependent RNA polymerase (RDRP) of ZIKV NS5 protein. Additionally, aloperine reduced viremia in mice and effectively lowered the mortality rate in infected mice. These findings highlight the potency of aloperine and its ability to target ZIKV infection, suggesting its potential as a promising antiviral drug against ZIKV.<\/jats:p>","DOI":"10.1002\/jmv.28913","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T10:20:26Z","timestamp":1688638826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The in vitro and in vivo antiviral effects of aloperine against Zika virus infection"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3755-4037","authenticated-orcid":false,"given":"Peiwen","family":"Zhou","sequence":"first","affiliation":[{"name":"Laboratory Animal Center Guangzhou University of Chinese Medicine Guangzhou China"},{"name":"Foshan Institute of Medical Microbiology Foshan China"},{"name":"Guangdong Key Laboratory of Virology, Institute of Medical Microbiology Jinan University Guangzhou 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