{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:57:22Z","timestamp":1753869442134,"version":"3.41.2"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is unknown whether Torque Teno virus (TTV) DNA load monitoring could anticipate the development of infectious events in hematological patients undergoing treatment with small molecular targeting agents. We characterized the kinetics of plasma TTV DNA in patients treated with ibrutinib or ruxolitinib and assessed whether TTV DNA load monitoring could predict the occurrence of Cytomegalovirus (CMV) DNAemia or the magnitude of CMV\u2010specific T\u2010cell responses. Multicenter, retrospective, observational study, recruiting 20 patients treated with ibrutinib and 21 with ruxolitinib. Plasma TTV and CMV DNA loads were quantified by real\u2010time PCR at baseline and days +15, +30, +45, +60, +75, +90, +120, +150, and +180 after treatment inception. Enumeration of CMV\u2010specific interferon\u2010\u03b3 (IFN\u2010\u03b3)\u2010producing CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T\u2010cells in whole blood was performed by flow cytometry. Median TTV DNA load in ibrutinib\u2010treated patients increased significantly (<jats:italic>p<\/jats:italic>\u2009=\u20090.025) from baseline (median: 5.76 log<jats:sub>10<\/jats:sub> copies\/mL) to day +120 (median: 7.83 log<jats:sub>10<\/jats:sub> copies\/mL). A moderate inverse correlation (Rho\u2009=\u2009\u22120.46; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) was found between TTV DNA load and absolute lymphocyte count. In ruxolitinib\u2010treated patients, TTV DNA load quantified at baseline was not significantly different from that measured after treatment inception (<jats:italic>p<\/jats:italic>\u2009\u2265\u20090.12). TTV DNA load was not predictive of the subsequent occurrence of CMV DNAemia in either patient group. No correlation was observed between TTV DNA loads and CMV\u2010specific IFN\u2010\u03b3\u2010producing CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T\u2010cell counts in either patient group. The data did not support the hypothesis that TTV DNA load monitoring in hematological patients treated with ibrutinib or ruxolitinib could be useful to predict either the occurrence of CMV DNAemia or the level of CMV\u2010specific T\u2010cell reconstitution; nevertheless, due to the small sample size, further studies involving larger cohorts are warranted to elucidate this issue.<\/jats:p>","DOI":"10.1002\/jmv.28933","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T11:34:55Z","timestamp":1688556895000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessment of the potential value of plasma Torque\u00a0Teno virus DNA load monitoring to predict cytomegalovirus DNAemia in patients with hematological malignancies treated with small molecule inhibitors: A proof\u2010of\u2010concept study"],"prefix":"10.1002","volume":"95","author":[{"given":"Carlos Solano","family":"de la 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