{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:22:49Z","timestamp":1790709769474,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rabies is a zoonotic viral disease characterized by an almost 100% fatality rate once symptoms appear. However, it can be prevented through timely postexposure prophylaxis (PEP). Currently, there is a growing trend to replace polyclonal rabies immune globulin (RIG) with monoclonal antibodies (mAbs) in rabies PEP. In this study, we developed a human bispecific antibody, GR1801, by combining two mAbs, A2 and B353, which target distinct epitopes. GR1801 is an asymmetric immunoglobulin G1 molecule, with one arm (A2 targeting epitope III) in fragment antigen\u2010binding (Fab) form and the other arm (B353 targeting epitope I) in single\u2010chain variable fragment (scFv) form, constructed using Knobs\u2010into\u2010Holes technology. GR1801 demonstrated the ability to neutralize 90 naturally occurring rabies virus (RABV) glycoprotein antigenic variants, 21 pseudotyped, and 18 live street RABVs, exhibiting broad\u2010spectrum neutralizing activity. In vivo, GR1801 provided protection equivalent to that of human RIG in golden hamsters challenged with lethal RABV. In conclusion, these findings demonstrate the neutralization potency and breadth of GR1801, which can be a promising candidate drug for rabies PEP, and a comprehensive testing against a broad spectrum of Chinese prevalent RABVs will be investigated in great detail in the future for the in vitro and in vivo studies.<\/jats:p>","DOI":"10.1002\/jmv.29016","type":"journal-article","created":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T21:38:21Z","timestamp":1692567501000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development of a novel bispecific antibody GR1801 for rabies"],"prefix":"10.1002","volume":"95","author":[{"given":"Caifeng","family":"Long","sequence":"first","affiliation":[{"name":"Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control, NHC Key Laboratory of Research on Quality and Standardization of Biotech Products NMPA Key Laboratory for Quality Research and Evaluation of Biological Products Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control, NHC Key Laboratory of Research on Quality and Standardization of Biotech Products NMPA Key Laboratory for Quality Research and Evaluation of Biological Products Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Beijing Wisdomab Biotechnology Co., Ltd. Beijing China"},{"name":"Antibody Research and Development Center Chongqing Genrix Biopharmaceutical Co., Ltd. Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanfei","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control, NHC Key Laboratory of Research on Quality and Standardization of Biotech Products NMPA Key Laboratory for Quality Research and Evaluation of Biological Products Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Feng","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun Jilin Province China"},{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchun","family":"Tu","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun Jilin Province China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou University Yangzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun Jilin Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Bian","sequence":"additional","affiliation":[{"name":"Antibody Research and Development Center Chongqing Genrix Biopharmaceutical Co., Ltd. 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Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0588-4899","authenticated-orcid":false,"given":"Lan","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control, NHC Key Laboratory of Research on Quality and Standardization of Biotech Products NMPA Key Laboratory for Quality Research and Evaluation of Biological Products Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.91"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2022.101204"},{"key":"e_1_2_10_4_1","unstructured":"World Health Organization. Rabies. 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