{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T07:46:04Z","timestamp":1776498364084,"version":"3.51.2"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T00:00:00Z","timestamp":1693785600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), the transcription template for all viral mRNAs, is highly stable and current treatment options cannot\u00a0effectively induce its clearance. Previously, we established an HBV persistence mouse model based on a clinical isolate (termed BPS) and identified interleukin\u201021\u00a0(IL\u201021) as a potent inducer of HBV clearance. Lipid nanoparticle (LNP) mediated delivery of mRNA has proven to be a highly safe and effective delivery platform. This work explored the applicability and effectiveness of the\u00a0mRNA\u2010LNP platform in IL\u201021\u2010based HBV therapies. First, LNP\u2010encapsulated murine IL\u201021 mRNA (LNP\u2010IL\u201021) was prepared, characterized, and demonstrated to engender IL\u201021 expression in vitro and in vivo. Next, LNP\u2010IL\u201021 was shown to induce clearance of both serum and intrahepatic HBV antigen and DNA in two HBV persistence mouse models based on BPS and recombinant cccDNA (rcccDNA), respectively, which was associated with HBV\u2010specific humoral and cellular immune responses. Furthermore, peripheral blood mononuclear cells from BPS persistence mice treated ex vivo with LNP\u2010IL\u201021 and HBV surface antigen (HBsAg) could induce similar HBV clearance upon infusion into recipient mice. These findings indicated that IL\u201021 combined with mRNA\u2010LNP platform represents a valid and promising strategy for developing novel therapeutics against chronic HBV infection.<\/jats:p>","DOI":"10.1002\/jmv.29062","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T13:11:44Z","timestamp":1693833104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Lipid nanoparticle\u2010mediated delivery of IL\u201021\u2010encoding mRNA induces viral clearance in mouse models of hepatitis B virus persistence"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8566-9149","authenticated-orcid":false,"given":"Zhongliang","family":"Shen","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Department of Infectious Diseases, National Medical Center for Infectious Diseases, Shanghai 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2466-3736","authenticated-orcid":false,"given":"Qirong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Department of Infectious Diseases, National Medical Center for Infectious Diseases, Shanghai Institute of Infectious Diseases and Biosecurity, Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (Ministry of Education\/National Health Commission\/Chinese Academy of Medical Sciences), Department of Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College Fudan University  Shanghai 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