{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:08:18Z","timestamp":1787314098580,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rabies, a lethal zoonotic encephalitis, remains a significant global health concern, causing an estimated 60\u2009000 annual fatalities worldwide. Dogs serve as the primary reservoirs and vectors for transmitting this infection to humans. Definitive diagnosis of rabies in both human and animal cases necessitates laboratory testing involving various clinical specimens. However, the complexity of laboratory infrastructure and the need for skilled personnel, along with the challenge of maintaining cold\u2010chain integrity during sample referral, hinder the decentralization of diagnostic facilities. This study aimed to assess the efficacy of the Truenat rabies assay, a rapid, portable, semiautomated, and closed PCR\u2010based system, for the diagnosis of rabies in both humans and animals. The Truenat assay demonstrated a sensitivity of 100% and a specificity of 86.96% when compared with the fluorescent antibody test (FAT), as the reference standard, on 147 canine brain samples tested. Notably, the Truenat assay exhibited a sensitivity and specificity of 100% when tested on 48 human brain specimens. Furthermore, an examination of 148 human antemortem samples (cerebrospinal fluid, saliva, and skin biopsy) using both the Truenat assay and a validated real\u2010time reverse transcriptase PCR assay revealed a\n                    <jats:italic>\u03ba<\/jats:italic>\n                    value of 0.505, indicative of a moderate level of agreement between the two tests. Thus, the Truenat assay offers a robust, reliable, and affordable point\u2010of\u2010care solution to enhance rabies diagnostic capacity in endemic areas.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29110","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T09:07:47Z","timestamp":1695200867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of a rapid, chip\u2010based, micro\u2010PCR assay for detection of rabies virus in human and canine specimens"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6124-9809","authenticated-orcid":false,"given":"Lonika","family":"Lodha","sequence":"first","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2018-7066","authenticated-orcid":false,"given":"Ashwini M.","family":"Ananda","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arya","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathya Priya","family":"Manuel","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujatha Valagere","family":"Sannaiah","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Mahadevan","sequence":"additional","affiliation":[{"name":"Department of Neuropathology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6039-1187","authenticated-orcid":false,"given":"Reeta S.","family":"Mani","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences  Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.91"},{"key":"e_1_2_9_3_1","unstructured":"World Health Organization (WHO) Food and Agriculture Organization of the United Nations (FAO) and World Organization for Animal Health (OIE). (2019).Zero by 30: the global strategic plan to end human deaths from dog\u2010mediated rabies by 2030: United Against Rabies Collaboration: first annual progress report: global strategic plan to end human deaths from dog\u2010mediated rabies by 2030. Accessed March 31 2023.https:\/\/apps.who.int\/iris\/handle\/10665\/328053"},{"key":"e_1_2_9_4_1","unstructured":"National Centre for Disease Control. National Action Plan for Dog Mediated Rabies Elimination from India by 2030. 2021. Accessed March 31 2023.https:\/\/ncdc.gov.in\/WriteReadData\/linkimages\/NationalActiopPlan.pdf"},{"key":"e_1_2_9_5_1","unstructured":"World Health Organization. (1992).WHO Expert Committee on Rabies [\u200emeeting held in Geneva from 24 to 30 September 1991]\u200e: eighth report(WHO Technical Report Series 824). Accessed March 22 2023.https:\/\/apps.who.int\/iris\/handle\/10665\/39308"},{"key":"e_1_2_9_6_1","unstructured":"World Health Organization. (2018).WHO Expert Consultation on Rabies: WHO TRS N\u00b01012. Accessed July 5 2023.https:\/\/www.who.int\/publications\/i\/item\/WHO-TRS-1012"},{"key":"e_1_2_9_7_1","first-page":"88","volume-title":"WHO Laboratory Techniques in Rabies","author":"Dean D","year":"1996"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23814"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21547"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11070649"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005010"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004776"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12669"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007128"},{"key":"e_1_2_9_15_1","unstructured":"World Health Organization. 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