{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:52:32Z","timestamp":1791287552909,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T00:00:00Z","timestamp":1696636800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Management of cytomegalovirus (CMV) in transplant patients relies on measuring plasma CMV\u2010loads using quantitative nucleic acid testing (QNAT). We prospectively compared the automated Roche\u2010cobas\u00ae6800\u2010CMV and Roche\u2010CAP\/CTM\u2010CMV with laboratory\u2010developed Basel\u2010CMV\u2010UL54\u201095bp, and Basel\u2010CMV\u2010UL111a\u201077bp. Roche\u2010cobas\u00ae6800\u2010CMV and Roche\u2010CAP\/CTM\u2010CMV were qualitatively concordant in 142\/150 cases (95%). In\u2010depth comparison revealed higher CMV\u2010loads of the laboratory\u2010developed assay and correlated with smaller amplicon size. After calibration to the 1.WHO\u2010approved CMV international standard, differences were reduced but remained significant. DNase\u2010I pretreatment significantly reduced CMV\u2010loads for both automated Roche\u2010CAP\/CTM\u2010CMV and Roche\u2010cobas\u00ae6800\u2010CMV assays, whereby 90% and 95% of samples became undetectable. DNase\u2010I pretreatment also reduced CMV\u2010loads quantified by Basel\u2010CMV\u2010UL54\u201095bp and Basel\u2010CMV\u2010UL111a\u201077bp, but remaining detectable in 20% and 35%, respectively. Differences were largest for 110 samples with low\u2010level CMV\u2010DNAemia being detectable but not\u2010quantifiable by Roche\u2010cobas\u00ae6800\u2010CMV, whereby the smaller amplicon sizes yielded higher viral loads for concordant positives. We conclude that non\u2010encapsidated fragmented CMV\u2010DNA is the major form of plasma CMV\u2010loads also measured by fully\u2010automated platforms. Amplicons of &lt;150\u2009bp and calibrators are needed for reliable and commutable QNAT\u2010results. We hypothesize that non\u2010encapsidated fragmented CMV\u2010DNA results from lysis of CMV\u2010replicating cells and represent a direct marker of viral cell damage, which contribute to delayed viral load responses despite effective antivirals.<\/jats:p>","DOI":"10.1002\/jmv.29139","type":"journal-article","created":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T18:47:34Z","timestamp":1696790854000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Amplicon size and non\u2010encapsidated DNA fragments define plasma cytomegalovirus DNA loads by automated nucleic acid testing platforms: A marker of viral cytopathology?"],"prefix":"10.1002","volume":"95","author":[{"given":"Karoline","family":"Leuzinger","sequence":"first","affiliation":[{"name":"Clinical Virology University Hospital Basel Basel Switzerland"},{"name":"Transplantation &amp; Clinical Virology, Department Biomedicine University of Basel Basel 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