{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:18:14Z","timestamp":1789417094819,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Occult hepatitis B (HBV) infection (OBI), characterized by low viral loads, accounts for much of the risk of HBV transfusion\u2010transmitted infection. With anticore antibodies (anti\u2010HBc) screening introduced in England, the imperative to identify OBI donors has increased. We aimed to develop an ultra\u2010sensitive PCR system and investigate risk factors for HBV DNA presence in blood donations. Seven extraction methods and three PCR assays were compared. The optimal system was sought to determine HBV DNA presence in anti\u2010HBc\u2010positive donations. Predictors of DNA positivity were subsequently investigated. Extraction from 5\u2009mL of plasma increased sample representation and resulted in HBV DNA detection in low viral load samples (~0.5\u2009IU\/mL). Screening of 487\u2009763 donations in 2022 identified two OBI donors and 2042 anti\u2010HBc\u2010positive donors, 412 of the latter with anti\u2010HBs\u2009&lt;\u2009100\u2009mIU\/mL. Testing of 134 anti\u2010HBc\u2010positive donations utilizing the 5\u2009mL extraction method identified two further HBV DNA\u2010positive donations. Higher anti\u2010HBc titer and anti\u2010HBs negativity were significant predictors of DNA detectability in anti\u2010HBc\u2010positive donations. An ultrasensitive PCR assay identified potentially infectious donations increasing HBV DNA detection in anti\u2010HBc\u2010positive donors from 0.5% to 1.9%. Anti\u2010HBc titers may further complement the risk stratification for DNA positivity in anti\u2010HBc screening and minimize unnecessary donor deferral.<\/jats:p>","DOI":"10.1002\/jmv.29144","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:07:46Z","timestamp":1696522066000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Ultrasensitive PCR system for HBV DNA detection: Risk stratification for occult hepatitis B virus infection in English blood donors"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3108-7917","authenticated-orcid":false,"given":"Michael X.","family":"Fu","sequence":"first","affiliation":[{"name":"Nuffield Department of Medicine University of Oxford Oxford 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